摘要
目的观察溶血卵磷脂(LPC)及辛伐他汀干预对体外培养人肝细胞(L-20)SRB I表达的影响。方法培养的肝细胞随机分为正常组、LPC损伤组及辛伐他汀组。分别用逆转录聚合酶链式反应(RT-PCR)、免疫细胞化学和蛋白质免疫印迹(W esternb lot)方法观察肝细胞SRB I基因和蛋白表达的变化。结果LPC损伤组肝细胞SRB I基因和蛋白表达水平较正常组增强(P<0.05);辛伐他汀组肝细胞SRB I基因和蛋白表达水平均高于LPC损伤组(均P<0.05)。结论LPC损伤肝细胞后,SRB I基因和蛋白表达水平轻度上调;辛伐他汀干预可明显上调体外培养的肝细胞SRB I表达。这一作用有利于胆固醇的逆向转运及脂质代谢的改善。
Objective To observe the impact of lysophosphatidyl choline （LPC） on the expression of scavenger receptor class B type I （SRBI） in L-20 cells and the impact of simvastatin. Methods Cultured hepatocytes were randomly assigned to normal group, LPC-damaged group and simvastatin group. The changes of mRNA and protein expression of SRBI in hepatic tissue were assayed by RT-PCR, immunocytochemistry and Western blot, respectively. Results The expression of SRBI was higher in LPC-damaged hepatocytes than that in normal cells at both mRNA and protein level （P 〈 0.05）. The hepatocytes of simvastatin group showed higher levels of mRNA and protein expression of SRBI as compared with LPC-damaged hepatocytes （P 〈 0.05）. Conclusions The expressions of SRBI mRNA and protein in LPC-treated hepatocytes are increased compensatively. Simvastatin treatment can significantly up-regulate the expression of SRBI mRNA and protein in cultured hepatocytes, which is effective for the contra-transport of cholesterol and the metabolism of lipid.
出处
《中国医师杂志》
CAS
2006年第3期345-347,共3页
Journal of Chinese Physician
