摘要
目的研究紫杉醇能否诱导卵巢癌HO-8910细胞凋亡,以及凋亡诱导与细胞周期改变的关系。方法紫杉醇不同浓度、不同作用时间分别处理HO-8910细胞,在光镜和电镜下观察紫杉醇作用后细胞形态变化,流式细胞仪分析细胞凋亡及细胞周期的改变。结果荧光染色及电镜观察到典型的凋亡细胞形态,流式细胞仪DNA含量分析,癌细胞在紫杉醇作用下,首先表现为G2/M期阻滞。但只有出现G2/M期明显阻滞后,经过一定时间才出现凋亡。结论紫杉醇能够诱导HO-8910细胞凋亡,紫杉醇诱导HO-8910细胞凋亡与细胞分裂期阻滞密切相关,这一作用机制将为临床治疗提供理论依据。
Objective To investigate whether paclitaxel can efficiently induce the apoptosis of ovarian cell HO-8910, and to study the relationship between the apoptosis of cells and the cell cycle. Methods With the treatment of paclitaxel with different concentrations and different time, the morphologic change of HO-8910 ovarian ceils was observed using fluorescence microscopy and transmission electron microscopy （TEM）, and the apoptosis of cells and the changes of cell cycle were determined by flow cytometry （FCM）. Results The typical changes of HO-8910 cell apoptosis were observed by TEM and Fluorescence microscopy. With the treatment of paclitaxel, the HO-8910 ovarian ceils were firstly arrested in G2/M phase, and the typical uhrastructural changes of apeptosis were appeared only after the ceils were apparently arrested in G2/M phase. Conclusion Paclitaxel can induce the apoptosis of HO-8910 cells and the apoptosis is associated with the blockage of G2/M phase in cell cycle.
出处
《中国医师杂志》
CAS
2006年第3期322-324,共3页
Journal of Chinese Physician
