摘要
目的研究核转录因子-κB(nuc lear factor-κB,NF-κB)在TNF-α诱导培养的人脐静脉内皮细胞凋亡过程中的作用。方法体外培养人脐静脉内皮细胞,并用10 ng/m l的TNF-α进行诱导,不同时间段观察NF-κB活性、NF-κB抑制物IκBα表达以及细胞凋亡的情况,EMSA测定NF-κB活性,W estern-b lot检测IκBα的表达情况;Tunel法检测细胞凋亡;并用NF-κB的抑制剂PDTC预处理细胞后来观察TNF-α诱导细胞凋亡的情况。结果TNF-α以时间依赖性诱导人脐静脉内皮细胞凋亡,NF-κB的活性在处理后10 m in开始增强,2 h后恢复正常,IκBα的表达在10m in开始下降,2 h恢复正常;PDTC能抑制TNF-α诱导的凋亡。结论TNF-α在诱导体外培养的人脐静脉内皮细胞时,IκBα降解,NF-κB激活,从而引起细胞的凋亡。
AIM To study the role of nulear factor-κB （NF-κB） in endothelial cell apoptosis induced by TNF-α. METHODS The cultured endothelial cells were treated with 10 ng/ml TNF-α in absence and presence of PDTC （an inhibitor of NF-κB）, the activity of NF-κB was measured by ellectrophoretic mobility shift assay （EMSA）, the expression of IκBα was analyzed by western blotting and apoptosis of cells was evaluated by TUNEL. RESULTS TNF-α induced apoptosis of endothelial cells in a time-dependant manner. The activity of NF-κB began to increase and the expression of IκBα began to decrease at 10 min after treatment with TNF-α. PDTC inhibited the apoptosis induced by TNF-α. CONCLUSION TNF-α activates NF-κB through phosphorylation of IκBα and thus induces apoptosis of endothelial cells.
出处
《心脏杂志》
CAS
2006年第2期152-154,共3页
Chinese Heart Journal
基金
云南省科委自然科学基金项目资助(No.2001C0070W)
