摘要
目的:建立新西兰兔营养性高脂血症模型,观察营养性高脂血症对兔外周血L-肉毒碱水平以及血液流变学的影响。方法:实验于2005-03/05在解放军南京军区南京总医院动物实验中心进行。纳入刚离乳非纯系新西兰兔36只,分为实验组(n=20)和对照组(n=16),分别给予高脂饲料和普通全价饲料喂养至3月龄,建立高脂血症动物模型。用全自动生化仪测定外周血总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇水平;采用HPLC法测定外周血中L-肉毒碱水平;同时用全自动血液流变分析系统分析血液流变学变化,并分析L-肉毒碱水平与总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇相关性。结果:共纳入动物36只,在实验过程中,对照组兔子3只、实验组1只因腹泻等原因死亡,进入结果分析数为32只。①实验组20只新西兰兔,有18只成功建立了营养性高脂血症动物模型,成功率为90%。②实验组总胆固醇、三酰甘油和低密度脂蛋白胆固醇相对于对照组,有极为显著的升高[(31.27±3.84),(0.70±0.11);(2.01±1.19),(0.53±0.14);(17.42±4.58),(0.22±0.05)mmol/L;P<0.01];对照组L-肉毒碱水平极显著的高于实验组[(58.89±7.76),(17.77±1.91)μmol/L,P<0.01]。③血液流变学各指标中,实验组全血黏度显著高于对照组[(1.95±0.23),(1.70±0.14)mPa·s,t=2.761,P<0.05],实验组红细胞聚集指数、红细胞计数与红细胞压积极显著的低于对照组[(5.24±0.34),(6.27±0.37);(3.94±0.36),(4.74±0.54)×1012L-1;(0.34±0.03),(0.44±0.02)L/L;t=-3.781～-7.735,P<0.01],其他各项指标在两组间无差异。④对照组的总胆固醇、三酰甘油和低密度脂蛋白胆固醇与L-肉毒碱水平呈正相关,高密度脂蛋白胆固醇与L-肉毒碱呈负相关,实验组的总胆固醇、三酰甘油和低密度脂蛋白胆固醇与L-肉毒碱水平呈负相关,高密度脂蛋白胆固醇与L-肉毒碱呈正相关。结论:幼年起摄入大量高脂食物极易诱发营养性高脂血症,并能显著降低外周血L-肉毒碱水平,出现失代偿,同时亦可影响血液流变学指标。
AIM： To establish the nutritional hyperlipemia model with New Zealand rabbits, in order to observe the effect of nutritional hyperlipemia on the level of L-camitine in the perpheral blood （PB） and blood theology.
METHODS： The experiment was carried out in the experimental animal center of Nanjing General Hospital of Nanjing Military Command between March and May 2005. Totally 36 non-clonal New Zealand rabbits just weaned were divided into experimental group （n=20） and control group （n=16）, which were fed with high fat forageand common complete forage until 3 months old respectively to establish the hyperlipemia animal models. The level of total cholesterol （TC）, triglyceride （TG）, high density lipoprotein cholesterol （HDL-C） and low density lipoprotein cholesterol （LDL-C） in the PB was measured with automatic biochemical analyzer; the L-camitine in the PB was determined with HPLC method; the changes of blood rheology was analyzed with the automatic blood rheometer to explore the correlation between the level of L-carnitine, TC, TG, HDL-C and LDL-C. RESULTS： Totally 32 rabbits were involved in the result analysis with 3 rabbits in the control group and 1 in the experimental group died of diarrhea or other reasons. ①The nutritional hyperlipemia models were established successfully in eighteen rabbits of the 20 ones of the experimental group with a rate of success 90%. ②The level of TC, TG and LDL-C in the experimental group were obviously increased compared with the control group [（31.27±3.84）, （0.70±0.11）; （2.01±1.19）, （0.53±0.14）; （17.42±4.58）, （0.22±0.05） mmol/L; P 〈 0.01]; the level of L-carnitine in the control group was obviously higher than that in the experimental group [（58.89±7.76）, （17.77±1.91） μmol/L,P 〈 0.01]. ③In the indexes of blood rheology, the whole blood viscosity in the experimental group was significantly higher than that in the control group [（1.95±0.23）, （ 1.70±0.14）mPa·s,t=2.761 ,P 〈 0.05], the aggregative index of red blood cell, red blood cell count and hematocrit were obviously lower than that in the control group [（5.24±0.34）, （6.27±0.37） ; （3.94±0.36）, （4.74±0.54）×10^12 L^-1; （0.34±0.03）, （0.44±0.02） L/L;t=-3.781-7.735 ,P 〈 0.01], and there were no significant differences in other indexes between the two groups. ④In the control group, the TC, TG and LDL-C were positively correlated with the L-carnitine, and the HDL-C was negatively correlated with the L-carnitine; While in the experimental group, the TC, TG and LDL-C were negatively correlated with the L-carnitine, and the HDL-C was positively correlated with the L-carnitine.
CONCLUSION： Massive high fat dietary taken since juvenile can easily induce nutritional hyperlipemia and significantly decrease the level of L-carnitine in PB, which can cause decompensation and influence the indexes of blood rheology.
出处
《中国临床康复》
CAS
CSCD
北大核心
2006年第16期55-57,共3页
Chinese Journal of Clinical Rehabilitation
