摘要
目的研究大鼠牙周炎牙及其正常牙在正畸移动中整合素β3 mRNA的表达变化。方法选用10周龄雄性成年SD大鼠96只,随机分为正常牙组、牙周炎牙组,每组48只。分别在加力后0 d、12 h、1 d、3 d、5 d、7 d每一时间点处死8只动物,制备标本。采用原位杂交检测牙周炎牙与正常牙正畸移动后β3mRNA转录水平的变化。结果正常牙组和牙周炎牙组加力后12 h和3 d时的牙周膜内破骨细胞整合素β3mRNA阳性强度均较加力前有明显增加(P< 0．001);其余时间点未见整合素β3mRNA阳性表达。在加力各时间点,两组整合素β3mRNA的表达强度无显著性差异(P>0．05)。结论在正常牙及牙周炎牙移动早期,牙周膜和牙槽骨骨髓腔内有整合素β3mRNA表达,提示整合素β3可能参与破骨细胞前体向破骨细胞转化及破骨细胞迁移和粘附。
Objective To study the integrin β3 mRNA changes after orthodontic treatment on normal teeth and periodontitis teeth in rats. Methods 96 adult SD rats of 10 weeks old were randomly divided into normal tooth move-ment group and periodontitis tooth movement group. The rats in the two groups were sacrificed after 0 d, 12 h, 1 d, 3 d, 5 d and 7 d of tooth movement. The alveolar specimens were prepared. The integrin β3 mRNA were detected using in situ hybridization in the specimens. The OD index of positively stained osteoclasts for integrin β3 mRNA after orthodontic tooth movement in the two groups were measured and compared. Results There were weak positive signals on the cytoplasm of osteoclasts in periodontum in both groups after 12 hours and 3 days force activation. No positive signals were detected in the rest samples. There was no difference in the OD of positive stained osteoclasts between normal and periodontitis groups. Strong expressions were present on cells with one or two nuclei in the alveolar marrow. Conclusion It is suggested that integrin β3 mRNA is related with osteoclasts maturation and migration in orthodontic tooth movement.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2006年第2期106-109,共4页
West China Journal of Stomatology
基金
高等学校博士学科点专项科研基金资助项目(20020610063)
