摘要
目的研究蛋白酶体亚基RC2在失神经骨骼肌中的变化规律及其意义。方法将48只SD大鼠,建立右下肢腓肠肌失神经支配模型,随机分为8组,每组6只,术后0 d为对照组,术后1、2、3、4、5、6、14 d为实验组；分别采用实时定量PCR和Western blot检测RC2在转录和蛋白水平的变化规律。结果RC2的mRNA从失神经后第1天开始表达上调,3 d达到高峰,为对照组的4．98倍,随后逐渐下降,14 d时降为对照组的0．42倍；各实验组与对照组相比,差异有统计学意义(P＜0．05)。RC2在蛋白水平的变化与转录水平有着相同的趋势,但是变化幅度较转录水平为低。失神经后3 d达到高峰,为对照组的2．70倍,随后缓慢下降,14 d时降为正常侧的O．35倍；失神经术后1、6 d与对照组相比,差异无统计学意义(P＞0．05)。2～5、14 d与对照组相比,差异有统计学意义(P＜0．05)。结论蛋白酶体亚基RC2在失神经骨骼肌早期表达增强,提示泛素-蛋白酶体途径在失神经骨骼肌早期的蛋白快速降解中占有重要地位。
Objective To observe expression of proteasome subunit RC2 in denervated skeletal muscles, and investigate the molecular mechanism of skeletal muscle wasting. Methods A model of gastrocnemius muscle denervation at the right lower limb was established in 48 rats. They were divided into 8 groups with 6 each. The subsequent changes in RC2 mRNA and protein levels were determined by real- time quantitative PCR and Western blot, respectively. Results Expression of proteasome subunit RC2 mRNA in rat denervated gastrocnemius muscle was upregulated gradually, peaked in 3 days, then decreased （4.98 folds and 0.42 fold at day 3 and day 14, respectively; P 〈 0.05）. The changes of protein levels were similar to that of mRNA levels, but the amplitude of changes was lower （2.70 folds and 0.35 fold at day 3 and day 14, respectively; P 〈 0.05）. Conclusion After denervation, expression of proteasome subunit RC2 in skeletal muscle was upregulated. This supports the concept that denervationinduced muscle atrophy is, at least in part, regulated by the ubiquitin-proteasome proteolytie pathway.
出处
《中华手外科杂志》
CSCD
北大核心
2006年第2期124-126,共3页
Chinese Journal of Hand Surgery
基金
国家自然科学基金(30371463)
关键词
肌萎缩
肌
骨骼
蛋白酶体
去神经支配
Muscle atrophy
Muscle, skeletal
Proteasome
Denervation
