摘要
目的寻找一种快速简便的分离毛囊干细胞的方法,探讨β-连环蛋白(-βcatenin)信号分子在毛囊干细胞增殖分化中的调节作用。方法利用毛囊干细胞黏附性强的特点,通过将毛囊细胞悬液黏附于包被有人胶原的平板而使富集毛囊干细胞分离。利用毛囊干细胞标志物Keratin 19(K19)及体外集落形成能力进行鉴定。采用免疫细胞化学及原位杂交技术检测分选后的毛囊干细胞-βcatenin的表达情况。结果毛囊干细胞悬液黏附于人胶原20 min后,K19的表达增加1.6倍,集落形成能力增加3.5倍。-βcatenin在细胞核中的蛋白表达增加。结论快速黏附法可以使富集毛囊干细胞分离,-βcatenin参与了毛囊干细胞的增殖及分化调控。
Objective To establish a convenient method for the selection of hair follicle stem cells and study the effects of β-catenin on the regulation of proliferation and differentiation of hair follicle stem cells. Methods Based on the strong specific adherent ability of stem cells, hair follicle cells were incubated in the plates coated with human collagen before use. Hair follicle stem cell＇s marker K19 and clone forming efficency were used to identify the stem cells.The expression of β-catenin was tested by immunocytochemistry and in situ hybridization. Results After adherence to collagen for 20min, the number of K19 positive cells among quickly-adhered cells increased 1.6 times more than unselected cells and clone forming efficiency increased 3.5 times.The expression of β-catenin protein increased in the nuclear of quickly-adhered cells. Conclution The selection method of human hair follicle stem cells by quick-adherence to collagen was established for the first time. β-catenin was involved in the regulation of hair follicle stem cells＇ proliferation and differentiation.
出处
《解剖学报》
CAS
CSCD
北大核心
2006年第2期153-157,共5页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(30170859)
高等学校博士学科点专项科研基金资助项目(20010023027)
关键词
毛囊
干细胞
Β-连环蛋白
免疫细胞化学
原位杂交
人
Hair follicle
Stem cell
β-catenin
Immunocytochemistry
In situ hybridization
Human
