摘要
目的探讨凋亡相关基因 bcl-2、Fas 在胸腺瘤伴重症肌无力患者瘤组织中的表达状况及其临床意义。方法经手术治疗的25例胸腺瘤伴重症肌无力患者的肿瘤组织标本为病例组,25例先天性心脏病患者手术时切取的正常胸腺组织标本为对照组,通过免疫组化的方法检测两组标本中 Bcl-2和 Fas 蛋白的表达水平。结果胸腺瘤中 Bcl-2及 Fas 表达水平均显著高于对照组,经 Ridit分析两者差异均有统计学意义(U 值分别为2.645、3.200,P 均<0.05),但 Bcl-2和 Fas 的表达水平与胸腺瘤患者的重症肌无力 Ossermen 分型、术前病程、年龄及性别等临床因素均无显著相关。结论Bcl-2和 Fas 在胸腺瘤伴重症肌无力的发病中可能具有重要作用。
Objective To evaluate the clinical significance of expression levels of apoptosisassociated genes, bcl-2 and Fas in thymoma tissue from patients with myasthenia gravis. Methods The study included 25 patients who underwent maximal thymectomy because of concurrent thymoma and myasthenia gravis. Clinical staging （Osserman classification） showed stage Ⅰ in 6,Ⅱ A in 3, ⅡB in 13 and Ⅲ in 3 cases. According to the WHO classification of thymomas（ 1999 ）, 5,3,7,7 ,and 3 patients had type A,AB,B1 ,B2,and B3 ,respectively, and no patient had type C. As a control group, the normal thymic tissue from 25 patients with congenital heart diseases was studied. The expression level of Bcl-2 and Fas in the tissue of thymoma and normal thymus was detected by immunohistochemistry. Results Bcl-2 and Fas expressions were significantly higher in thymomas compared with normal thymus by Radit analyses （ U = 2. 645, P 〈 0. 05 and U = 3. 200, P 〈 0. 05, respectively）. The expression level of Bcl-2 and Fas showed no relation to the course of the disease, age, Osserman classification and sex. Conclusion The result suggests that the two apoptosis-associated factors, Bcl-2 and Fas, may play important roles in the pathogenesis of thymoma concurrent with myasthenia gravis.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2006年第4期240-242,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
