摘要
本文研究了不同因子对芦笋（ＡｓｐａｒａｇｕｓｏｆｆｉｃｉｎａｌｉｓＬ．）悬浮细胞生长及其多糖含量的影响，结果表明：５％蔗糖和０．１ｇ／４０ｍｌ干重细胞接种量可促进其细胞生长；但不加谷氨酰胺和５％蔗糖有利于其多糖含量的提高。综合细胞生长和多糖含量两方面情况，以５％蔗糖、０．１ｇ／４０ｍｌ干重细胞接种量、７５ｍｇ／Ｌ的谷氨酰胺、５０ｍｇ／Ｌ的肌醇以及５ｍｇ／Ｌ的２，４－Ｄ为佳．
Effects of different factors on the growth of suspension cells of Asparagus officinalis and on its polysaccharide content have been studied in this paper. The results showed that 5% succose in the liquid medium and the inoculated amount of 0. 1 gram dry cells per 40ml liquid medium greatly promoted the asparagus cell growth and that none glutamine in the liquid medium and the inoculated amount of 0.1 gram dry cells per 40ml liquid medium were beneficial to the increase of asparagus cell polysaccharide content.Considered from both asparagus cell growth and its polysaccharide content, the best combination was the inoculated amount of 0.1 gram dry cells per 40ml with 5% sucrose, 75mg/L glutamine, 50mg/L inositol and 5mg/L 2,4-D in media.
关键词
细胞生长
多糖
石刁柏
Asparagus
Cell growth
Polysaccharride content
