摘要
目的探讨甘草酸对犬心脏停跳复苏后脑复苏的影响及其机制。方法 18只健康杂种犬随机分为3组(n=6):对照组(A组)、心脏复跳后常规治疗组(B组)、心脏复跳后甘草酸治疗组 (C组),A组开胸后不作处理,术毕观察8 h;B组心脏停跳18 min,复跳10 min时静脉输注20％甘露醇 5 ml·kg-1和地塞米松1 mg·kg-1,复跳6 h时追加半量,并根据血气结果静脉输注适量碳酸氢钠,术毕观察8 h;C组复跳10 min时静脉输注甘草酸注射液40 ml·kg-1,其余处理同B组,术毕观察8 h。术后 8 h进行神经功能评分(PBSS),随即处死动物取脑组织,测定线粒体膜流动性、Na+-K+-ATPase、Ca2+- ATPase、Mg2+-ATPase活性以及脑皮质丙二醛(MDA)含量和脑皮质含水率。结果与A组比较,B组脑皮质MDA含量、脑皮质含水率和线粒体膜荧光偏振度、微粘度、各向异性均增高(P<0．05或 0．01),而PBSS评分、Na+-K+-ATPase和Mg2+-ATPase活性降低(P<0．01)。与B组比较,C组PBSS评分、Na+-K+-ATPase和Mg2+-ATPase活性增高(P<0．01),而脑组织MDA、脑含水率和脑线粒体膜荧光偏振度、微粘度、各向异性降低(P<0．05或0．01)。结论甘草酸可改善心脏停跳复苏后脑复苏,可能与提高脑线粒体ATP酶活性,抑制脂质过氧化反应,保护线粒体膜流动性有关。
Objective To study the effects of glycyrrhizin on cerebral mitochondrial ATPase and membrane fluidity and malondialdehyde （MDA） and water content and brain function after cardiac arrest and resuscitation. Methods Eighteen dogs weighing 10-14 kg were randomly divided into 3 groups （ n = 6 each） ： group A control; group B cardiac arrest and resuscitation and group C glycyrrhizin ＋ cardiac arrest and resuscitation, The animals were anesthetized with fentanyl, intubated and mechanically ventilated and PaCO2 was maintained within normal range. The chest was opened. In group B and C cardiac arrest was produced by clamping of ascending aorta and coronary peffusion with hyperkalemic cardioplegic solution and maintained for 18 min and resuscitated by direct cardiac massage, adrenaline and defibrillation. The animals were observed for 8 h after spontaneous cardiac rhythm resumed. In group C glycyrrhizin injectio 40 ml·kg^-1 was infused over 8 h as soon as spontaneous cardiac rhythm resumed. Brain function was evaluated according to Pittsburgh Brain stem score （PBSS）. The animals were then killed and their brains removed for determination of （1） mitochondrial membrane fluidity and Na^＋ -K^＋ -ATPase and Mg^2＋ -ATPase activity and （2） brain MDA and water content. Results The mitochondrial membrane viscosity and cerebral MDA and water content were significantly higher and ATPase activity was significantly lower in group B （cardiac arrest） than in group A （control）. Brain function was also impaired by global cerebral ischemia-repeffusion （I/R） in group B. In group C glycyrrhizin infusion significantly attenuated the deleterious effects of cerebral I/R by reducing mitochondrial membrane viscosity and cerebral MDA and water content and increasing ATPase activity. Glycyrrhizin infusion also improved brain function. Conclusion Glycyrrhizin can ameliorate the deleterious effects of global cerebral I/R induced by cardiac arrest.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2006年第2期163-165,共3页
Chinese Journal of Anesthesiology
关键词
甘草酸
脑缺血
再灌注损伤
心肺复苏术
Glycyrrhizic acid
Brain ischemia
Reperfusion injury
Cardiopulmonary resuscitation
