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用ELISA检测稻飞虱的捕食性天敌 被引量:7

Dectection of Rice Planthopper Remains in Predatory Insects and Spiders by ELISA
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摘要 利用酶联免疫吸附试验(ELISA)方法测定了不同种类的捕食性天敌的阳性反应率.发现稻飞虱不同发育阶段的蛋白质组成一致,所有捕食性天敌种类中以食虫沟瘤蛛。 An ELISA which was used to detect and quantify ingested rice planthoppers in arthropod predators collected from paddy fields was developed. The detection limit of the assay was about one hundredth of an homogenised adult or 3 ̄5th instar nymph rice planthopper. Tests with 2 species of rice planthopper( Sogatella furcifera and Nilaparvata lugens ) showed that those which had been used as the principal immunogens reacted most strongly in the assay and no evidence of significant cross reaction was found with each other or with any other species and with rice material. Among tested predatory species, Ummeliata insecticeps, Pirata subpiraticus , clubionid, and salticid had the highest positive reaction rates.
出处 《中山大学学报(自然科学版)》 CAS CSCD 1996年第5期119-122,共4页 Acta Scientiarum Naturalium Universitatis Sunyatseni
基金 国家自然科学基金 广东省自然科学基金资助项目
关键词 ELISA 检测 稻飞虱 天敌 捕食性天敌 detection, rice planthopper, predator, ELISA
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