摘要
目的观察菟丝子水提物对活性氧(ROS)所致人精子膜结构和功能氧化损伤的干预作用及其对精子膜功能的保护作用。探讨菟丝子治疗男性不育弱精子症的作用机制。方法采用Percoll梯度离心法优选具有正常生理功能的精子作为正常精子模型,将精子悬液分为正常组、模型组、阳性药对照组(维生素C组)和菟丝子水提物小、中、大剂量组,应用次黄嘌呤-黄嘌呤氧化酶(HX-XO)体系产生ROS,在有氧环境下,不同剂量(每1 mL含0.125,0.25和0.5 g生药)的菟丝子水提物与精子悬液共同孵育后,检测精子膜脂质过氧化损伤程度,通过精子低渗膨胀试验评估精子膜功能,并与已知的抗氧化剂维生素C对照。结果菟丝子水提物小、中、大剂量组(每1 mL含0.125,0.25和0.5 g生药)在相同的条件下均可提高精子悬液超氧化物歧化酶(SOD)活力,降低丙二醛(MDA)含量,对ROS所致精子膜的损伤均具有不同程度的干预作用,对精子膜功能具有一定的保护作用。其中每1 mL含0.125,0.5 g生药的菟丝子水提物组与维生素C组(0.25 g.L-1)无明显差别(均P>0.05),0.25 g生药的菟丝子水提物组明显优于维生素C组(P<0.001)。结论适量的菟丝子水提物能显著提高精子悬液SOD活力,降低MDA含量,通过其抗氧化作用干预ROS对精子膜的脂质过氧化反应,对精子膜结构和功能具有明显的保护作用,这可能是菟丝子治疗男性不育弱精子症的作用机制之一。
OBJECTIVE To study the intervention by extract from Cuscuta chinensis in the structure of human sperm membrane and the oxidative injury of function from active oxygen （ROS） and the protection of the function of human sperm membrane. And to study the mechanism about Cuscuta chinensis treating male＇s infertility and asthenoospermia. METHODS To select sperm with normal physiological function using the Percoll gradient centrifugation for the normal sperm model, and separate the sperm suspension into the normal group, the model group and the control group （Vitamin C group）, and the massive, the medium and the small dose of Cuscuta chinensis. The ROS was made from hypoxanthine-xanthine oxidase（HX-XO）, and ROS, different content（0.125, 0.25,0.5 g· L^-1） of extract were hatched with sperm in the oxygen environment, the sperm membrane lipid peroxide injury were analyzed, and the function of sperm membrane were analyzed by sperm hypoosmoticswelling（HOS）. RESULTS In the same conditions, the small, medium and massive extract from Cuscuta chinensis （0.125,0.25, 0.5 g·L^- 1 ） improved SOD vitality of sperm suspension, reduced the content of MDA, intervened the injury of sperm membrane by ROS to some extent and protected some function of sperm membrane. The 0.125 and 0.5 g· L^- 1 extract had no obvious difference（ P 〉 0.05） with Vitamin C（0.25 g· L^- 1 ） in it, but the content（0.25 g· L^- 1 ） of extract is significantly better than control VitC（ P 〈 0.001 ）. CONCLUSION the suitable content of extract from Cuscuta chinensis can significantly improve the SOD vitality of sperm suspension, reduce the content of MDA, and intervene lipin peroxidation by ROS in sperm membrane by the way against oxidation, significantly protect the structure and function of sperm membrane, that is one of the mechanism for Cuscuta chinensis treating male＇ s infertility and asthenoospermia.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2006年第7期515-518,共4页
Chinese Pharmaceutical Journal
基金
浙江省中医药科研基金项目(2003C070)
杭州市科技发展计划项目(2004433Q15)
关键词
菟丝子
活性氧
精子
脂质过氧化作用
Cuscuta chinensis
active oxygen
sperm
lipin peroxidation
