摘要
为了加快小麦抗性亲本的选配,培育具有持久抗性的小麦新品种,根据大多数已克隆的植物抗病基因所编码蛋白产物的NBS-LRR(核苷酸结合位点和富含亮氨酸区域,N ucleotide b ind ing s ite-leuc ine richrepeat)和STK(丝氨酸/苏氨酸蛋白激酶,Ser/T hr K inase)保守结构域,合成24条简并引物,对四川省主要利用的82份小麦育种抗性亲本材料进行PCR扩增和聚丙烯酰胺凝胶电泳检测,筛选出多态性高的三对引物组合:P tok in1N/P tok in1N、C er3 l/XLRR INV 2、A S3/S2,进一步扩增,共获得123条带,其中54条带具有多态性,多态率达43.9%。U PGM A分析把82份材料分为两大类,其中LB 0485、C 866-1、R 116、R 185为I类,其余78份材料为Ⅱ类;Ⅱ类材料在遗传距离0.397处又分为Ⅱ-1和Ⅱ-2两个亚类。分析结果表明:(1)82份小麦供试材料间存在丰富的抗病基因同源序列的多样性,聚类分析结果与相应的抗性鉴定结果基本吻合;(2)国外引进的抗性材料与四川省广泛使用的小麦育种亲本材料的抗性背景不同,两者之间能够很好地区分开来;(3)R系材料具有广泛的抗性遗传基础,在大多数类群中均有分布。
Most known plant disease-resistance （R） genes include nucleotide binding site （NBS）or leucinerich repeats （LRRs） and serine/threonine protein kinase （STK） in their encoded products domains. Twenty-four sets of degenerate oligonucleotide primers were designed based on these conserved domains. By polymerase chain reaction （PCR） and denatured polyacrylamide-gel electrophoresis techniques, disease resistance gene analogs have been amplified on 82 wheat materials which mainly used in Sichuan province. We selected three sets of primers （Ptokin 1 N/Ptokin 2 N, Cer31/XLRRINV2, AS3/S2）, which amplified total 123 bands, 54 of the 123（43.9%） bands showed polymorphic for further study. UPGMA cluster analysis proposed the 82 wheat materials could be divided into two groups on the 0. 457 genetic distance （GD）, four of Chinese wheat including LB0485, C866-1, Rl16 and R185 was clustered into group Ⅰ. The others （78 varieties） was clustered into group Ⅱ, which consisted of group Ⅱ-1, which comprised two introduced German materials, and group Ⅱ-2, which comprised 66 Chinese wheat and 10 foreign wheat. This result demonstrated the R fines spreading around in most subgroups and the distinct difference between foreign wheats and Chinese wheats. The UPGMA analysis compared with the result of infection type against the disease （powdery mildew） in fields, showed that the clustering conformed the resistance performance. And the pedigree analysis result was better accordance with the field resistance reactions. All of the result indicated RGAP （resistance gene analog polymorphism） technique provides an useful and efficient way to improve the efficiency of parent materials selection in wheat breeding and to accelerate the process of developing wheat cultivars with durable resistance.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2006年第2期21-26,共6页
Journal of Triticeae Crops
基金
四川省科技厅应用基础项目(03NG002-008)
教育厅重点项目(2004A020)
关键词
小麦
抗病基因同源序列
聚类分析
Wheat
Resistance gene analogs （RGAs）
Cluster analysis
