期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

细胞质型APX的纯化、抗体的制备及放线菌素D对APX活性的影响 被引量：2

Purification of Cytosolic APX from Barley Leaves,Preparation of Antibodies and Effect of AMD on APX Activities
在线阅读 下载PDF
导出
摘要 利用硫酸铵分步沉淀与过柱层析提纯大麦叶片细胞质型APX(Ascorbate peroxidase,抗坏血酸过氧化物酶,EC1.11.1.11),SDS-PAGE电泳显示其是分子量约为29 kD的单体.将纯化的APX免疫青紫蓝兔,获取多抗,Westernblot证实科品7号、鉴4的细胞质型APX具有免疫交叉性.盐处理后耐盐性不同的品种叶片APX的含量发生变化,APX含量变化的规律与盐胁迫下APX活性变化的规律相似,说明盐胁迫下APX含量的变化是引起其活性变化的主要原因之一.不同浓度的AMD(Actinomycin D,放线菌素D)处理均能抑制盐和非盐胁迫下大麦叶片的APX活性. Ascorbate peroxidase （APX, EC1. 11.1.11 ） was purified to homogeneity from barley （ Hordeum vulgare L. ） leaves. APX was determined as a monomer with a molecular mass of about 29 kD by SDS- polyacrylamide gel electrophoresis. Western blot analysis using polyclonal antibodies raised against barley APX revealed that there exists immune cross-reaction of APX between Jian 4 and Kepin 7, and APX contents of two barley cuhivars differing in NaCI sensitivity changed in the course of NaCl stress. Changes in APX contents showed the same trends as that of APX activities. APX activities in leaves of barley seedlings were inhibited by Actinomycin D （AMD） at different concentration.
作者 陈沁 王伟 刘友良
机构地区 上海大学生命科学学院 南京农业大学生命学院
出处 《上海大学学报（自然科学版）》 CAS CSCD 北大核心 2006年第2期211-215,共5页 Journal of Shanghai University:Natural Science Edition
关键词 APX 抗体 WESTERN BLOT 盐胁迫 大麦 放线菌素D(AMD) ascorbate peroxidase （APX） antibody Western blot NaCl stress barley actinomycin D（AMD）
分类号 S641.3 [农业科学—蔬菜学]
  • 相关文献

参考文献18

  • 1ASADA K.Ascorbate peroxidase-a hydrogen scavenging eneyme in plants[J].Physiol Plant,1992,85:235-241.
  • 2MOCK H P,KEETMAN U,KRUSE E,et al.Defense responses to tetrapyrrole-induced oxidative stress in transgenic plants with reduced uroporphyrinogen decarboxylase or corproporphyrinogen oxidase activity[J].Plant Physiol,1998,116:107-116.
  • 3MITTLER R,ZILINSKAS B A.Purification and characterization of pea cytosolic ascorbate peroxidase[J].Plant Physiol,1991,97:962-968.
  • 4王爱国.植物生理与分子生物学[M].北京:科学出版社,1998.366-389.
  • 5TANAKA K,SUDA Y,KONDU N,et al.O3 tolerance and the ascorbate-dependent H2O2 decomposing system in chloroplasts[J].Plant Cell Physiol,1985,26:1425-1431.
  • 6LIANG Yongchao,CHEN Qin,ZHANG Wenhua.Exogenous silicon (Si) increases antioxidant enzyme activity and reduces lipid peroxidation in roots of slat-stressed barlet (Hordeum vulgare L.)[J].J Plant Physiol,2003,160:1157-1164.
  • 7陈沁,刘友良.谷胱甘肽对盐胁迫大麦叶片活性氧清除系统的保护作用[J].作物学报,2000,26(3):365-371. 被引量：86
  • 8ZHANG Wenhua,CHEN Qin,LIU Youliang.Relationship between H^+ -ATPase activity and fluidity of tonoplast in barley roots under NaCl stress[J].Acta Bot Sin,2002,44:292-296.
  • 9NAKANO Y,ASADA K.Purification of ascorbate peroxidase in spinach chloroplasts; its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical[J].Plant Cell Physiol,1987,28(1):131-140.
  • 10MIYAKE C,CAO W H,ASADA K.Purification and molecular properties of thylakoid-bound ascorbate peroxidase in spinach chloroplast[J].Plant Cell Physiol,1993,34(6):881-889.

二级参考文献5

共引文献166

同被引文献20

  • 1孙卫红,王伟青,孟庆伟.植物抗坏血酸过氧化物酶的作用机制、酶学及分子特性[J].植物生理学通讯,2005,41(2):143-147. 被引量：116
  • 2孙学成,谭启玲,胡承孝,甘巧巧,易长城.低温胁迫下钼对冬小麦抗氧化酶活性的影响[J].中国农业科学,2006,39(5):952-959. 被引量：66
  • 3李惠华,赖钟雄.植物抗坏血酸过氧化物酶研究进展(综述)[J].亚热带植物科学,2006,35(2):66-69. 被引量：25
  • 4Nakano Y,Asada K. Hydrogen peroxide is scavenged by ascorbate - specific peroxidase in spinach chloroplasts [ J ]. Plant Cell Physiol, 1981,22:867 - 880.
  • 5Chen Z, Su W, Tang Z C. Heat protective role and mechanism of heat shock protein Hpc60 [ J ]. Chinese Sci Bulletin, 2000,45 ( 2 ) : 161 - 164.
  • 6Hemandez J A, Olmose E, Corpas F J, et al. Salt induced oxidative stress in chloroplasts of pea plants [ J ]. Plant Sci, 1995,105 : 151 - 167.
  • 7Schoner S, Krause G H. Protective system against active oxygen species in spinach : response to cold acclimation in excess light [ J ]. Planta, 1990,180:383 - 389.
  • 8Mittler R,Feng X, Cohen M. Post - transcriptional suppression of cytosolic ascorbate peroxidase expression during pathogen - induced programmed cell death in tobacco[ J ]. Plant Cell, 1998,10:461 -473.
  • 9Murgia I,Tarantino D,Vannini C,et al.Arabidopsis thaliana plants overexpressing thylakoidal ascorbate peroxidase show increased resistance to Paraquat-induced photooxidative stress and to nitric oxide-induced cell death[J].Plant Journal,2004,38(6):940-953.
  • 10Sarowar S,Kim E N,Kim Y J,et al.Overexpression of a pepper ascorbate peroxidase-like 1 gene in tobacco plants enhances tolerance to oxidative stress and pathogens[J].Plant Science,2005,169(1):55-63.

引证文献2

二级引证文献10

上海大学学报（自然科学版）
2006年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部