摘要
应用免疫组化ＡＢＣ法，对２９例子宫内膜非典型增生和３２例子宫内膜高分化腺癌进行了Ⅳ型胶原、ＣＥＡ及乳铁蛋白对比检测。结果显示：非典型增生基底膜Ⅳ型胶原阳性表达为２５／２９．阳性物呈连续线状包绕腺体；高分化腺癌阳性表达为８／３２，阳性物为间断不连续的片段。作者对Ⅳ型胶原、ＣＥＡ及乳铁蛋白这三种免疫标记物在鉴别非典型增生与高分化腺癌中的意义作了分析。并对这两类病变各自的主要超微结构特点作了观察及讨论。
he ABC immunobistochemical metheod was performed on 29 cases of emdometrial atypicalhyperplasia(AH)and 32 cases of well differentiated carrcinoma(WDCA)to detect collagenⅣ,CEAand lactoferin,The results indicated tbat 25/29 AH cases had intact epithelial basementmembranes with Linear staining of collagen Ⅳ,and 8/32 WDCA leasions had disrupted basementmembranes with fragment staining of collagen Ⅳ. CEA, Lactoferrin strong staining were foundmainly in WDCA cells, with weak reaction seen in AH cells.The reslts surggest that the collagen Ⅳ detection is of practical value in the differential diagnosisbetween AH and WDCA,Ultrastructurally,most carcinomatous cells exhibited poorly developedmocrovilli and eXtreme polymorphous nuclei.The basal lamina disruption can be easily identified. In AH cells, the nucrovilli were numerous and well developed, and the neuclear pleomorphism wasnot obvious,The basal lamina of AH presented as a continuing line.
出处
《重庆医科大学学报》
CAS
CSCD
1996年第2期107-110,共4页
Journal of Chongqing Medical University
关键词
子宫内膜肿瘤
子宫内膜增生
非典型
电镜
腺癌
Endometrial adenocarroinoma
Endometrial atypicalhyperplasia
Immunohistoch emistry
Electmmicroscopy
