摘要
应用短期人工瘤胃发酵技术,通过接种不同数量的瘤胃液,确定瘤胃液数量(微生物数量)对人工瘤胃发酵产气量及pH值的影响。试验分为10个处理,即缓冲液与瘤胃液体积比分别为11∶、21∶、31∶、41∶、51∶、61∶、71∶、81∶、91∶和101∶。每个处理设置2个重复,同时每个处理设置2个空白。将样品发酵48h,分别在2、4、8、12、24、48h6个时间点记录注射器活塞的位置,并在发酵结束时测定发酵液的pH值。结果表明:不同处理组2h和4h的产气量与瘤胃液比例极相关(P<0.01),8h和12h的产气量与瘤胃液比例显著相关(P<0.05),24h以后(包括24h)的产气量与瘤胃液比例不相关(P>0.05)。瘤胃液比例对人工瘤胃发酵的pH值没有影响。
The short-term rumen simulation technique inoculated with different rumen fluid level was used to study effect of rumen fluid （rumen micro-organism） level on gas production and pH in the artificial rumen. Ten treatments, i. e., artificial saliva： rumen fluid by 1：1, 2：1, 3：1, 4：1, 5：1, 6：1, 7：1, 8：1, 9：1, 10：1 were used. Each treatment had 2 replicates and 2 blanks. Gas production was recorded after 2, 4, 8, 12, 24 and 48 h of fermentation, respectively, and pH was measured at the end. Results showed that gas production in the first 12 h had significant correlations with the rumen fluid level, especially that within the beginning 4 h, while gas production over 12 h and pH are irrelative to rumen fluid level.
出处
《中国畜牧杂志》
CAS
北大核心
2006年第5期39-41,共3页
Chinese Journal of Animal Science
