摘要
通过对52例肺癌患者、8例肺部良性疾病患者和10名健康人血清游离DNA含量与肿瘤标志物（TM）CA19-9、CA125、CYFRA21-1、CEA和NSE的联合检测，对两种检测结果作相关性分析。将表皮生长因子受体（EGFR）作为肿瘤基因标志物，运用荧光定量RT-PCR方法检测血清游离DNA含量。结果显示，10名健康人血清游离DNA含量平均为18．81ng／mL（范围：0．17～54.64ng／mL）。若以19ng／mL作为血清游离DNA含量的阳性临界值，则87．5％的健康人低于此水平。8例肺部良性疾病患者游离DNA含量均值为76．86ng／mL（范围；5．33～189．7ng／mL），其中4例（50％）高于阳性临界值。52例肺癌患者游离DNA均值为107．6ng／mL（范围：6．39～1617ng／mL），37例（71．2％）的肺癌患者血清游离DNA含量高于正常值。血清游离DNA含量诊断肺癌的价值等同于肿瘤标志物五项指标联合检测。其灵敏度、特异性、准确性分别为：71．15％，50％，68．3％，提示血清游离DNA含量作为一项新颖肿瘤标志物具有潜在的临床诊断价值。
To evaluate the diagnostic value and relationship between five tumor markers （CA19- 9,CA125 ,CYFRA21-1,CEA,NSE） and free DNA in serum for lung cancer detection and try to find a new and more efficient tumor marker, the amounts of CA19-9, CA125, CYFRA21-1, CEA, NSE were determined by RIA and free DNA was determined by the use of quantitative real time PCR amplification of the human epidermal growth factor receptor （EGFR） in 52 lung cancer patients and 8 cases of benign pulmonary disease and 10 healthy controls. The resulls showed that average concentration of free DNA in serum of lung cancer patients, benign pulmonary disease and healthy controls was 107.6ng/mL, 76.86ng/mL and 18.8ng/mL, respectively. The diagnostic sensitivity, specificity and accuracy of free DNA for lung cancer were 71. 2%, 50% and 68.3%, same as the diagnostic value of combined detection of five tumor markers. The sensitivity, specificity and accuracy of the five tumor markers and free DNA combinend detection for lung cancer were 94.2%, 25% and 85%, respectively. The free DNA in the serum of lung cancer patients may be a new and better tumor marker.
出处
《标记免疫分析与临床》
CAS
2006年第1期7-10,共4页
Labeled Immunoassays and Clinical Medicine
关键词
游离DNA
表皮生长因子受体
肿瘤标志物
荧光定量RT-PCR
肺癌
Free DNA
Epidermal growth factor receptor
Tumor marker
Real-time quanti- tative polymerase chain reaction
Lung cancer
