摘要
通过采取石膏固定和失神经两种制动方式对大鼠骨骼神经生长因子(NGF)含量的动态观察,以及骨组织中的碱性磷酸酶(AKP)、抗酒石酸酸性磷酸酶(TRAP)活性的检测,探讨失神经因素与固定因素在导致大鼠骨质疏松过程中的不同作用。实验将10周龄SD雄性大鼠随机分为对照组、失神经手术组、固定组,于1d、10d、30d、60d后处死,ELISA双抗体夹心法测定骨组织中NGF含量,化学比色法测定AKP、TRAP活性。结果表明:NGF检测显示失神经组与固定组间比较,10d、60d两组含量明显增高(P<0.01),且大鼠在失神经和固定情况下的变化规律明显不同;AKP活性检测显示,30d固定组、10d失神经组、1d固定组降低明显(P<0.01);TRAP活性检测显示,10d失神经组、30d固定组明显降低(P<0.01),仅有10d固定组部分升高(P<0.05)。失神经和固定在导致大鼠骨量下降或骨质疏松时,骨质的丢失在时程上存在明显的差异,其发生的机制有着本质的区别。失神经和固定制动两种方法导致骨质疏松的特点都是低转换型。
Through observing the dynamic change of nerve growth factors ano cnecking up the activity of alkaline phosphatase （AKP）,tartrate-resistant acid phosphatase （TRAP） of bone organization with the method of gesso and denervation immobilization,this paper discusses the different effect of the denervation and fixed factor in the course of osteoporosis. In this experiment, 10-month-old SD male rats are randomly divided into control group ,denerved group and immobilized group. The rats are killed respectively after 1d, 10d, 30d and 60d. NGF is measured by the method of ELISA and AKP. The TRPA is measured by chemical colorimetry. The result shows that denerved group compared with fixed group, the NGF of 10d and 60d group increase obviously. Moreover ,the changing rule is different obviously under the condition of denervation and immobilization. AKP and TRAP activity of fixed group which are killed after 30d and denerved group which are killed after 10d increase obviously. Osteoporosis is different on the time course when denerved and immobilized factor has the same function to cause the decrease of bone quantity and osteoporosis. The character of denervation and immobilization leading to osteoporosis is both low conversion types.
出处
《中国体育科技》
CSSCI
北大核心
2006年第2期128-131,共4页
China Sport Science and Technology
关键词
神经生长因子
碱性磷酸酶
抗酒石酸酸性磷酸酶
骨代谢
失神经
鼠
动物实验
nerve growth factor
alkaline phosphatase
tartrate-resistant acid phosphatase
bone metabolism
denervation
rat
animal experiment
