摘要
目的：从分子水平观察肝细胞癌组织ＨＢＶＤＮＡ和癌基因的表达情况及与发病的关系。方法：应用原位核酸分子杂交技术研究４５例原发性肝细胞癌和２５例肝硬化的石蜡切片组织中ＨＢＶＤＮＡ和ｎ－ｒａｓ、ｃ－ｍｙｃ癌基因表达情况。结果：在肝细胞癌中见有ｎ－ｒａｓ和ｃ－ｍｙｃ表达增强，其检出率分别为６８．９％（３１／４５）和４０．０％（１８／４５），高于肝硬化组（２８．０％，８．０％）（Ｐ＜０．０５）。４０％肝细胞癌病例（１８／４５）两种癌基因同时过量表达，４５例肝细胞癌组织中有１９例存在有ＨＢＶＤＮＡ序列（４２．２％）并均具有ｎ－ｒａｓ或ｃ－ｍｙｃ过度表达。结论：两种癌基因ＨＢＶＤＮＡ可能有激活癌基因作用，肝细胞癌发生中可能起着重要的协同作用。ＨＢＶ感染与肝细胞癌发生有密切关系。
Methods:The expression of HBV DNA and oneogene n-ras,c-myc in paraffin embedded liver tissue from 45 cases of hepatocellular carcinoma(HCC)and 25 cases of liver cirrhosis had been studied by in situ hybridization with photobiotin labeled probes.Results:The detection rates of n-ras and c-myc were 68.9%(3l/45)and 40.0%(18/45)respectively in HCC,which were obviously higher than the expression of n-ras and c-myc in tissues from liver cirrhosis(P<0.05).In HCC group,18 out of 45 patients(40.0%)were positive for n-ras and c-myc simultaneously.It implied that the cooperating activity of n-ras and c-myc might be responsible for the malignant phenotypic alteration in some or most cases of HCC.The detection rates of HBV DNA was 42.2%(19/45)in HCC.Conclusion:The overexpression of n-ras or c-myc was found in all HBV DNA positive cases.These results confirm the close association between HCC and HBV infection.
