摘要
目的了解深圳地区宫颈炎患者高危型人乳头瘤病毒(HPV)的感染情况。方法以荧光PCR技术同时应用两种检测不同数量型别的试剂盒检测100例(宫颈炎组77例和正常对照组23例)宫颈分泌物标本。结果100例妇女宫颈分泌物样本中,宫颈炎组和正常对照组高危型HPV感染率分别为16.9%和4.3%。两种试剂检测结果不一致的标本用DNA测序法检测均证实存在高危型HPV感染。结论宫颈炎患者高危型HPV感染率较高,正常人也有感染。荧光PCR技术检测高危型HPV与DNA测序法结果一致。
Objective To investigate the high-risk human papilloma virus（HPV） infection among the patients with cervicitis in Shenzhen. Methods 77 samples of cervical secreta from the patients with cervicitis and 23 samples from healthy women as a control were detected by fluorescent PCR assays with two kinds of kits. Results Among 100 cervical secreta specimens, the infectious ratio was 16.9% in the cervicitis group and with 4.3% in the controls. The high-risk HPV infection were verified by DNA sequencing from the different results detected with two kits. Conclusions It is suggested that women with cervicitis have higher high-risk HPV infection rate than that of healthy women. The detected results are correspondence between fluorescent PCR and DNA sequencing methods.
出处
《江西医学检验》
2006年第1期19-20,93,共3页
Jiangxi Journal of Medical Laboratory Sciences
