摘要
目的:研究姜黄素对双侧颈总动脉永久性结扎(2VO)所致血管性痴呆大鼠学习记忆功能减退的作用。方法:以2VO来制备大鼠血管性痴呆模型,将大鼠分为3组(假手术组、溶剂对照组、给药组),一部分于术后2周检测脑组织氧自由基(O2.-)和羟自由基(.OH),另一部分于术后4周和8周分别进行水迷宫实验,来检测各组大鼠学习记忆能力的差异,通过HE染色和免疫组化来观察细胞数量和形态的差异。结果:姜黄素能明显降低因2VO所造成的氧自由基(O2.-)和羟自由基(.OH)水平的升高(P<0.05),改善由2VO所致的学习记忆功能的减退(P<0.05和P<0.01)。HE染色和免疫组化证实,姜黄素减少了2VO所导致的神经细胞死亡。结论:姜黄素能改善2VO所致的学习记忆减退,其机制与减少自由基的产生以及减少神经细胞死亡有关。
Objective： To investigate the effects of curcumin on spatial learning and memory of vascular dementia rats induced by a permanent bilateral ligation of common carotid arteries （2VO）. Methods： SD rats were randomly divided into sham-operated, vehicle-treated and curcumin-treated group. The levels of O2^- and OH were detected at 2 weeks after the surgery. The morris water maze test was performed to detect the effects of curcumin on spatial learning and memory of vascular dementia rats at 4 weeks and 8 weeks after the surgery. HE and immunocytochemistry were performed to investigate the cell number of CA1 subfeild of hippocampus. Results： Curcumin can significantly decrease the levels of O2^- and OH. The spatial learning and memory of vascular dementia rats were impaired and that of the curcumin-treated group was ameliorated. HE and immunocytochemistry proved the difference of the cell number of CA1 subfield of hippocampus. Conclusion： The results demonstrate that curcumin can alleviate the impairment resulted from vascular dementia by reducing the level of free radical and the cell death of CA1 subfield of hippocampus.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第4期246-248,261,F0002,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省高校自然科学基金资助项目(03KJB310083)
关键词
姜黄素
血管性痴呆
学习记忆
curcumin
vascular dementia
learning and memorizing
