摘要
目的：探讨变形链球菌粘附机制。方法：以变形链球菌ＮＧ８基因１０１７ｂｐ扩增产物作模板，采用不对称聚合酶链反应（ＰＣＲ）法扩增单链ＤＮＡ片段进行序列测定分析。结果：与文献报道的序列一致。
Aim: To study the mechanism of streptococcus mutans adhesion. Methods: Using 1 017bp amplified DNA fragments from NG 8 as template, generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the gene: spaP. Results: Similar to those described previously by Okahashi et al (1989). Conclusions: This group may provide new message for the mechanism of streptococcus mutans adhesion and these research of dental plaque.
出处
《牙体牙髓牙周病学杂志》
CAS
1996年第2期85-87,共3页
Chinese Journal of Conservative Dentistry
基金
全军"八五"科研基金题资助课题3091002
关键词
聚合酶链反应
链球菌属
表面抗原
口腔细菌学
polymerase chain reaction, DNA sequencing analysis, streptococcus , surface antigen, Gene
