摘要
人酸性成纤维细胞生长因子(haFGF)是一类对来源于中胚层和神经外胚层的多种类型的细胞具有广泛生物学活性的细胞生长因子。研究了乙酸浓度对重组人酸性成纤维细胞生长因子(rhaFGF)改构体表达体系Escherichia coliBL21(DE3)/pET3C-haFGF的生长和表达的影响,探索了几种高密度培养重组大肠杆菌的流加分批发酵技术。通过比较几种不同的补料策略间歇流加、间歇-静态DO平衡、静态溶氧平衡-葡萄糖饥饿法、静态pH,有效的避免了发酵过程中,尤其是诱导表达阶段乙酸的积累。菌体密度OD600nm=22左右,可溶性重组人酸性成纤维细胞生长因子纯化后水平为450mg/L。
Human acidic fibreblast growth factor（haFGF） was a kind of cell growth factor with wide bio-activity on cell from mesectoderm and neuro-ectoderm. In this paper, the effect of acetate concentration on the growth and expression of recombinant human acidic fibroblast growth factor mutant system E. coli BL21 （DE3）/pET3C-haFGF was investigated. Four fed-batch modes： batch-fed, batch-DO static balance, DO static balance-glucose starvation, and pH-static state were investigated. The accumulation of acetate during the fermentation course was effectively inhibited. The OD6oo^m value was about 22, after purification, the soluble rhaFGF yielded 450mg/L. During the fermentation, no special ways such as pure oxygen, pressure were adopted, thus the established process would be easily scaled up for industry purpose.
出处
《生物工程学报》
CAS
CSCD
北大核心
2006年第2期322-327,共6页
Chinese Journal of Biotechnology
基金
国家十五重大生物医药专题滚动立项资助(No.2004AA2Z3C60)。~~
