摘要
检测广州地区艾滋病患者中人类疱疹病毒8(HHV-8)的感染状况并完成部分序列的测序,从而了解HHV-8感染相关的Kaposi’s肉瘤在本地区艾滋患者中可能的罹患风险,并初步探讨HHV-8在本地区是否存在基因序列的变异。使用n-PCR法检测患者唾液中的HHV-8 DNA,PCR产物经ABI3100系统直接测序。结果显示在广州地区艾滋病患者中唾液HHV-8 DNA阳性率为20.0%,而在作为对照的健康组的阳性率为0.0%,艾滋病患者组与健康对照组间具非常显著性差异;检测的部分碱基序列未发现变异。提示在广州地区的艾滋病患者中存在较高的HHV-8感染。
In order to find out the human herpesvirus 8（HHV-8） infection in patients with AIDS in Guangzhou, to elucidate the possible risk of AIDS related Kaposi＇s sarcoma in the patients and to explore the possible geneticvariation of HHV-8 in this region, n-PCR was used to detect the HHV-8 DNA in the saliva of the patients with AIDS,and then the PCR products were sequenced by ABI 3100 system. Results showed that the HHV-8 positive rates in AIDS group and the control group were respectively 20.0 % and 0.0 %. The difference between the 2 groups was very significant. No DNA sequence difference was found in the 261 - 511 base range, as compared with that of the published one （NC 003409） . The HHV-8 infection rate was quite high in AIDS patients of Guangzhou region.
出处
《病毒学报》
CAS
CSCD
北大核心
2006年第2期123-125,共3页
Chinese Journal of Virology
基金
广东省医学科研基金项目(A2003395)
教育部"留学回国人员科研启动基金"(教外司留[2004]527号)
