摘要
背景:依据清宫秘方研制而成的纯中药制剂清宫长春丹胶囊,具有补肾益精、强筋壮骨、延缓衰老之功效,临床用于治疗身衰体弱、精力不足、健忘易倦、头晕耳鸣、腰痛膝酸等病症。目的:观察清宫长春丹胶囊对以肌肉注射氢化可的松制备的“阳虚”模型小鼠应激能力的影响。设计:完全随机对照动物实验。地点:承德医学院中药研究所(河北省中药研究与开发重点实验室)。材料:二级昆明种小鼠160只,,体质量19～21g,雌雄各半。方法:实验于2002-02/2002-12在承德医学院中药研究所药理毒理研究室完成。采用昆明种小鼠160只,雌雄各半,随机分为游泳耐力实验组(n=60)、常压耐缺氧实验组(n=50)、单核巨噬细胞吞噬功能组(n=50)3组,每组再平均分为长春丹0.25,0.5,1.0g/kg,模型对照组及正常小鼠对照组共5个亚组。禁食12h,以肌肉注射氢化可的松25mg/kg连续7d制备“阳虚”模型,按分组给不同剂量长春丹,给药容积20mL/kg,连续给药7d,模型对照组给予等容积生理盐水灌胃,最后一次给药40min后,分别进行小鼠游泳耐力实验、小鼠常压耐缺氧时间实验、小鼠单核巨噬细胞吞噬功能的实验(尾静脉注射印度墨汁0.lmL/kg,分别于注射后2min,10min从眼眶静脉丛取血20μL,计算单核巨噬细胞炭粒廓清指数K值),观察不同剂量的长春丹对小鼠游泳时间、耐缺氧时间和单核巨噬细胞吞噬功能的影响。结果:纳入实验的160只小鼠全部进入结果统计分析,无缺失值。①长春丹胶囊0.25,0.5,1.0g/kg剂量组及正常对照组小鼠游泳时间均明显长于模型对照组[(10.94±3.79),(13.68±5.62),(14.58±5.49),(16.12±2.35),(6.45±4.87)min;t=2.522,3.367,3.586,3.979,P<0.05～0.001]。②长春丹胶囊0.25,0.5,1.0g/kg剂量组及正常对照组小鼠常压耐缺氧时间均明显长于模型对照组[(19.45±4.63),(21.18±4.25),(22.58±5.21),(23.12±4.78),(12.35±4.89)min;t=1.566,2.104,2.004,2.303,P<0.05～0.01]。③长春丹胶囊0.25,0.5,1.0g/kg剂量组及正常对照组小鼠单核巨噬细胞吞噬功能炭粒廓清指数(K)均明显高于模型对照组(0.0236±0.0106,0.0291±0.0092,0.0308±0.0086,0.0318±0.0101,0.0125±0.0081;t=2.63,4.282,4.898,4.714,P<0.05～0.01)。结论:清宫长春丹胶囊灌胃小鼠游泳时间明显延长,且可剂量依赖的延长小鼠常压耐缺氧时间,提高小鼠单核巨噬细胞的吞噬功能,显著的增强“阳虚”小鼠的应激能力。
BACKGROUND： Qinggong changchun dan （QGCCD） capsule has efficacy in profiting kidney-yang and bettering muscle or bone ,it could treat debility, deficiency of energy, forgettery and tiredness and the ache of waist or knee in clinic.
OBJECTIVIg： To study the effect of QGCCD capsule on stress capability of mice with kidney-yang deficiency.
DESIGN： Completely randomized controlled trial.
SETTING： Institute of Traditional Chinese Medicine, Chengde Medical College （Key Laboratory of Research and Exploiture of Traditional Chinese Medicine in Hebei Province）.
MATERIALS： Totally 60 stirp Kunming mice, weighting 19-21 g, of either gender, of grade II, were selected in this study.
METHODS： The experiment was conducted in the Department of Pharmacology and Toxicology, Institute of Traditional Chinese Medicine of Chengde Medical College from February 2002 to December 2002. Totally 160 Kunming mice of either gender were randomly divided into three groups：Swimming endurance group （n=60）, tolerance of hypoxia at normal pressure group （n=50） and single macrophage function group （n=50）; and mice in each group were divided into 5 subgroups, including 0.25, 0.5, 1.0 g/kg QGCCD groups, model control group and normal control group. Mice were fasted for 12 hours and injected with 25 mg/kg hydrocortisone for 7 successive days to establish yang-deficiency model. Various dosages of QGCCD were given with the same volume of 20 mL/kg for 7 successive days. Mice in model control group were treated with the same volume of saline. Forty minutes later, testes of swimming tolerance, tolerance time of anoxia and engulfment capability of macrophage were performed on mice [To take 20μL blood in the eyepit vein plexus after the mice were injected 2 minutes and 10 minutes later respectively, and to count the charcoal particle expurgation index （K） of macrophage] to observe effect of various dosages of QGCCD on swimming tolerance, tolerance time of anoxia and engulfment capability of macrophage.
RESULTS： Data of totally 160 mice was entered the final analysis without any loss. ① Swimming times of mice in 0.25, 0.5, 1.0 g/kg QGCCD groups and normal control group were longer than those of mice in model control group [（10.94±3.79）, （13.68±5.62）, （14.58±5.49）, （16.12±2.35）, （6.45±4.87） minutes; t=2.522, 3.367, 3.586, 3.979, P 〈 0.05-0.001]. ② Tolerance time of anoxia of mice in 0.25, 0.5, 1.0 g/kg QGCCD groups and normal control group were longer than those of mice in model control group [（19.45 ±4.63）, （21.18±4.25）, （22.58±5.21）, （23.12±4.78）, （12.35±4.89） minutes; t=1.566, 2.104, 2.004, 2.303, P 〈 0.05-0.01]. ③ Engulfment capability of macrophage and Ki index of mice in 0.25, 0.5, 1.0 g/kg QGCCD groups and normal control group were higher than those of mice in model control group （0.023 6±0.010 6, 0.029 1±0.009 2, 0.030 8±0.008 6, 0.031 8 ±0.010 1, 0.012 5±0.008 1; t=2.63, 4.282, 4.898, 4.714, P〈 0.05-0.01）.
CONCLUSION： QGCCD can prolong the swimming time of mice obviously, prolong tolerance time of anoxia according to dosage dependence, increase engulfment capability of macrophag, and strengthen stress capability of mice with kidney-yang deficiency.
出处
《中国临床康复》
CSCD
北大核心
2006年第11期188-190,共3页
Chinese Journal of Clinical Rehabilitation
