摘要
AIM： To investigate the differently expressed genes in human colorectal adenocarcinorna.METHODS： The integrated approach for gene expression profiling that couples suppression subtractive hybridization, high-throughput cDNA array, sequencing, bioinformatics analysis, and reverse transcriptase real- time quantitative polymerase chain reaction （PCR） was carried out. A set of cDNA clones including 1260 SSH inserts amplified by PCR was arrayed using robotic printing. The cDNA arrays were hybridized with florescent-labeled probes prepared from RNA of human colorectal adenocarcinoma （HCRAC） and normal colorectal tissues.RESULTS： A total of 86 genes were identified, 16 unknown genes and 70 known genes. The transcription factor Sox9 influencing cell differentiation was downregulated. At the same time, Heat shock protein 10 KDis downregulated and Calmoulin is up-regulated.CONCLUSION： Downregulation of heat shock protein 10 KD lost its inhibition of Ras, and men attenuated the Ras GTPase signaling pathway, increased cell proliferation and inhibited cell apoptosis. Down-regulated transcription factor Sox9 influences cell differentiation and cell-specific gene expression. Down-regulated Sox9 also decreases its binding to calmodulin, accumulates calmodulin as receptor-activated kinase and phosphorylase kinase due to the activation of PhK.
瞄准：在人的肤色调查不同地表示的基因表面的腺癌。方法：为介绍那夫妇抑制的基因表示的综合途径减少性的杂交，高产量的 cDNA 数组，定序，生物信息学分析，和反向的 transcriptase 即时量的聚合酶链反应(PCR ) 被执行。包括 1260 SSH 插入的一套 cDNA 克隆由 PCR 放大了用机器的打印被穿。cDNA 数组是 hybridized，荧光灯标签的探针从表面的腺癌(HCRAC ) 和正常渲染的人的颜色的 RNA 准备了表面的纸巾。结果：86 基因的一个总数被识别， 16 未知基因和 70 已知的基因。影响细胞分化低是的抄写因素 Somult9 调整了。同时，热震惊蛋白质 10 KDis 在下面调整并且 Calmoulin 是起来调整的。结论：热吃惊蛋白质 10 KD 的 Downregulation 失去了它地岬的抑制，然后稀释了表明小径，增加的房间增长和禁止的房间 apoptosis 的地岬 GTPase。下面调整的抄写因素 Somult9 影响细胞分化和房间特定的基因表示。下面调整的 Somult9 也减少它的绑定到 calmodulin，由于 PhK 的激活作为激活受体的激酶和磷酸化酶激酶积累 calmodulin。
基金
Supported by postdoctoral fellowship from China National Human Affairs Ministry to Chen Yao,Development Program of Sichuan University to Chen Yao
China National Excellent Youth Fund to Zhong-Guang Zhou,No.39925032
