摘要
从PK-15细胞中提取总RNA,用RT-PCR方法扩增出猪F as基因,将其克隆到PM D 18-T载体上,进行序列分析。结果表明,克隆的猪F as基因序列与G enB ank上登录的猪F as基因同源性为100%,与人、牛、羊的F as基因核苷酸及推导的氨基酸序列同源性分别为73.4%、79.2%、76.4%和56.2%、67.0%、64.6%。F as蛋白胞内区的死亡域,其氨基酸序列在猪、人、牛和羊的F as基因中呈现较高的同源性。
The Fas gene was amplified from the total RNA from PK-15 cells using reverse transcription polymerase chain reaction （RT-PCR） and cloned into PMD18-T vector, and then sequenced. The sequence analysis of Fas gene showed that 100% shares homology with that of published in GenBank. The nucleotide and the deduced amino acid sequences of the Fas gene of pig shared 73.4%, 79.2%, 76.4% and 56.2%, 67.0%, 64.6% with those of human, bovine and sheep, respectively. The intracellular portion of Fas containing death domain was hight homology among of pig, human, bovine and sheep.
出处
《广西农业生物科学》
CAS
CSCD
2006年第1期6-10,共5页
Journal of Guangxi Agricultural and Biological Science
基金
广西科技攻关项目(桂科攻0428011-1)
广西大学科学技术研究重点基金资助项目(2003ZD01)
关键词
FAS
克隆
序列分析
Fas
cloning
sequence analysis
