摘要
目的建立简单差示蛋白法,获取两种海蛇的特征蛋白用作专属鉴别。方法采用SDS-PAGE与West-ern-blot结合分析并取差示蛋白直接测序。结果确认1号海蛇的特征蛋白为14.6kD及8.7kD两个组分,2号海蛇的特征蛋白为16.7kD及10.2kD两个组分,并完成14.6kD及10.2kD氨基酸测序。结论采用一维电泳结合抗原分析,方法简单,可以获取差示蛋白用于中药材基原鉴别。
Objective To identify the specific proteins in two hydrophis （1^# and 2^# ） with a simple differential proteins display method. Method By means of SDS - PAGE and Western blot, combined with analysis, the specific proteins in two hydrophis were obtained and been sequencing. Result The specific proteins of 1^# are ld. 6kD and 8.7kD components and of 2^# are 16.7kD. 10.2 kD components. The amino acid sequences of ld. 6 kD and 10. 2 kD components were assayed respectively. Conclusion Differential proteins can be obtained and used to identify the Chinese traditional medicinal crops by means of one - dimensional electrophoresis combined with antigen analysis.
出处
《中药新药与临床药理》
CAS
CSCD
2006年第2期120-122,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
