摘要
研究了不同浓度的柠檬酸钠、肝素钠、乙二胺四乙酸二钠（EDTA-Na2）和草酸钠对刺参Stichopus japonicus体腔细胞的抗凝效果。结果表明，3．8mg／mL的柠檬酸钠、1．0～1．5mg／mL的EDTA-Na2、0．2～0．6mg／mL的肝素钠、1．0～1．5mg／mL的草酸钠均能较好地保持体腔细胞的形态活性，适合用作刺参体腔细胞的抗凝剂。4种抗凝剂中EDTA-Na2的效果最好。
Effects of different levels of sodium citrate, EDTA - Na2, HEPES, and sodium oxalate on anticoag-ulation of coelomocytes were studied in sea cumcumber Stichopus japonicus. The results showed that the coelomocytes were morphologically kept activity at 1.0 - 1.5 mg/mL EDTA - Na2, 3.8 mg/mL sodium citrate, 0.2 - 0.6 mg/mL HEPES, and 1.0 - 1.5 mg/mL sodium oxalate, EDTA - Na2 being the best anticoagulant.
出处
《大连水产学院学报》
CSCD
北大核心
2006年第1期87-89,共3页
Journal of Dalian Fisheries University
基金
国家自然科学基金资助项目(30471323)
