摘要
以新疆野生盘羊(Ovis ammon)和萨能奶山羊转基因(GFP)成纤维细胞为核供体细胞,分别以绵羊卵母细胞和普通山羊卵母细胞为受体细胞进行了核移植,对不同种类核移植重构卵进行电融合处理,发现盘羊重构卵电融合参数为1.4 kV/cm、20μs/次、间隔1 s时的重构卵融合率最高;对于转基因(GFP)萨能奶山羊重构卵的处理,电融合前先放入融合液中于38.5℃下平衡5 min,融合时电融合参数为1.5 kV/cm、20μs/次、间隔1 s时的融合率最高。实验中发现将融合后的两种重构胚胎先置入IVM培养液中于38.5℃下平衡4 h,可降低重构胚胎的死亡率,提高发育质量和卵裂率。
In this study using the fibroblasts of Xinjiang wild Ovis ammon and the transgenic （GFP） fibroblasts of＇ Saanen dairy goat as donor cells and using the Abattoir-derived oocytes of domesticated sheep and goat as recipient cells the nuclear transfer experiments had been prcessed. During the electricfusion experiments of different kinds of nuclear transfer embryos the high fusion rate of Ovis am rnon cell-cytoplasts was found when the electricfusion parameter was 1.4 kV/cm for 20μsee. For the transgenic cell-cytoplasts of Saanen dairy goat, before electricfusion the cell-cytoplasts were first put into fusionsolution to be balanced for 5 min at 38. 5℃, when the electricfusion parameter was 1. 5 kV/cm for 20μsee, the high fusion rate could be obtained. After electricfusion these two kinds of reconstruction embryos were balanced at 38.5℃ in IVM medium for 4 hours can decrease the death rate of reconstruction embryos and increase the development quality and the cleavage rate of reconstruction embryos.
出处
《西北农业学报》
CAS
CSCD
北大核心
2006年第2期1-6,共6页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金项目(30471242)
中国科学院百人计划项目资助
关键词
转基因
核移植
电融合
重构胚胎
体细胞克隆
Transgenic
Nuclear transfer
Electrofusion
Reconstruction embryo
Somatic cloning
