摘要
向日葵籽苗下胚轴原生质体，培养在含有ＢＡ０．５ｍｇ／Ｌ，２，４－Ｄ０．５ｍｇ／Ｌ，ＮＡＡ０．１ｍｇ／Ｌ和葡萄糖０．５５ｍｇ／Ｌ的改良Ｋａｏ培养基中，２４～２８ｈ后，原生质体开始分裂。包埋在琼脂糖０．６％中的原生质体，培养５ｄ后，分裂频率达９５％以上。生长旺盛的小愈伤组织转移到含有２ｉｐ０．１ｍｇ／Ｌ，ＩＡＡ０．０１ｍｇ／Ｌ，腺嘌呤４０ｍｇ／Ｌ和ＧＡ３０．０１ｍｇ／Ｌ的Ｔｈｏｍｐｓｏｎ液体培养基上１３ｄ后，原生质体诱导的少数愈伤组织发生根分化。
Protoplasts isolated fromhypocotyls of Helianthus annuus seedlings grown in the dark were cultured on modified mediumafter Kao (1982) containing BA 0. 5 mg/L,2, 4-D 0. 5 mg/L, NAA 0.1 mg/L and glucose 0. 55 mol/L as the sole source of caralhydrates. Protoplasts started to divide after 24-28 h of culture and after five days 95% ofprotoplasts immobilized in 0. 6% agarose haddivided at least once. Rapidly growing microcalli were transferred to liquid medium afterThomPSon et al. (1986) containing 2-ip 0. 1mg/L, IAA 0. of mg/L, adenine 40 mg/Land GA3 0. 01 mg/L. A few calli derivedfrom protoplasts produced a root each after 13days of culture.
