摘要
目的在重组人骨形态发生蛋白-2(rhBMP-2)异位成骨过程中,局部应用神经生长因子(NGF),探讨NGF对BMP诱导成骨的作用。方法ICR小鼠36只,随机分为实验组和对照组,于右侧股后部肌间隙内植入rhBMP-2胶原复合物。术后第3天开始,连续7d,在实验组和对照组rhBMP-2植入部位,分别注射NGF和磷酸盐缓冲液(PBS)。术后第10、20和30天取材,进行放射学、生化和组织学检测。结果两组均于术后第10天有新骨形成,但实验组新骨生成量明显大于对照组;术后第10和第20天,实验组局部组织碱性磷酸酶活性明显高于对照组;术后各时间段实验组局部组织钙和磷含量均明显高于对照组;术后第30天,实验组骨组织胶原纤维排列明显较对照组规则。结论NGF具有协同rhBMP-2诱导成骨的作用。
Objective To investigate the effect of nerve growth factor （NGF） on the bone induction of recombinant human bone morphogenetic protein-2 （rhBMP-2） through local application of NGF in the osteoinductive process of BMP. Methods Thirty-six ICR mice were divided into the experimental group and control group at random, and rhBMP-2/collagen composite was implanted into the right thigh muscle pouch of each group. NGF or vehicle was daily injected into the implanted sites of BMP, respectively, for 7 clays starting from the third clay after surgery. At d10, d20 and d30 after implantation, new bone formation was measured radiographically, biochemically and histologically to compare the osteogenetic capacity of the two groups. Results In both groups, new bone formation was found at d10. However, there was significantly more new bone in the experimental group according to histological and radiographic examinations. At d10 and 20, alkaline phosphatase activity of the local tissue in the experimental group was significantly greater than that in the control group, and calcium and phosphonium contents of samples were also greater in the experimental group. Arrangement of collagen fibers became more regular in the experimental group than in the control group. Conclusion NGF possesses synergistic effect on ectopic bone formation induced by rhBMP-2.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2006年第3期273-277,共5页
Journal of Shanghai Jiao tong University:Medical Science
