摘要
目的通过检测结核性和癌性胸腔积液中IL-16水平,探讨其与T淋巴细胞亚群之间的关系。方法收集62例胸腔积液(32例结核性和30例癌性)患者的胸腔积液与静脉血,ELISA测胸腔积液上清液及血清中IL-16水平;流式细胞仪测胸腔积液中T淋巴细胞亚群;行胸腔积液中细胞计数及分类。结果62例胸腔积液者,胸腔积液中IL-16水平高于其血清水平。结核性胸腔积液中IL-16水平高于癌性胸腔积液中IL-16水平。胸腔积液中IL-16水平与淋巴细胞总数、T细胞数及CD4+细胞数呈正相关(r分别为0.526、0.638、0.701,P值均小于0.001)。结论与癌性胸腔积液相比,结核性胸腔积液中IL-16水平高,IL-16有可能在CD4+T细胞浸润到胸腔的过程中具有重要的趋化作用。
Objective Interleukin-16 （IL-16） is a chemoattractant of CD4^＋ lymphocytes, and it has been implicated in the pathogenesis of various inflammatory diseases. The aim of the present study was to measure IL-16 in pleural effusions caused by tuberculosis and malignancy and its relationship with cell and differential counts as well as lymphocyte subsets. Methods Pleural effusion and venous blood samples were collected from 32 patients with tuberculous pleuritis and 30 lung cancer patients with malignant effusion. Analysis of pleural effusion for total leukocytes and cell differentials of leukocytes was performed. Three-color flow cytometry was performed to determine T lymphocyte subsets in cell pellets of pleural effusion. The concentration of IL-16 in cell-free supernatants of pleural effusion and sera was measured by a sandwich enzyme-linked immunosorbent assay. Results In all the studied patients, the level of IL-16 was significantly higher in pleural effusion than in serum. The levels of IL-16 were significantly higher in tuberculous than in malignant effusions. In pleural effusion, positive correlations were found between the IL-16 levels and total cell counts, lymphocytes, CD~ T cells, as well as CD4^＋ T cells. Conclusions Compared to malignant pleural effusion, IL-16 appeared to be increased in tuberculous pleural effusion. The pleural effusion IL-16 levels were positively related to the numbers of CD4^＋ T cells, suggesting that IL-16 might be capable of inducing CD4^＋ T cell infiltration into pleural space.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2006年第3期199-201,共3页
Chinese Journal of Internal Medicine
基金
广西自然科学基金资助项目(0342031)
