摘要
目的分析3种常见乙型肝炎病人的血清蛋白谱特征。方法采用酶联免疫吸附实验检测乙肝标志物,双缩脲法检测总蛋白,溴甲酚绿法(BCG)检测白蛋白,琼脂糖凝胶电泳法分析血清蛋白组分。结果乙型肝炎病毒表面抗原阳性携带者与正常人血清蛋白各组分之间差异无显著意义(P>0·05);乙型肝炎患者与正常人血清蛋白各组分之间差异均有显著意义(P<0·01)。乙型肝炎患者HBsAg(+)、HBeAg(+)、HBcAb(+)组与乙型肝炎患者HBsAg(+)、HBeAb(+)、HBcAb(+)组血清蛋白各组分相比较,Alb呈显著降低(P<0·05);α1、α2、β差异无显著意义(P>0·05),γ球蛋白呈显著升高(P<0·05)。结论血清蛋白电泳、总蛋白、白蛋白联合检测可全面了解乙型肝炎患者血清蛋白谱的变化特征,对乙型肝炎的临床诊断和疗效观察有重要的参考价值。
Objective To analyze the characters of serum protein patterns of three kinds of patients with hepatitis B. Methods Determine the marker of hepatitis B by ELISA ,total protein by biuret method,albumin by bromocresol green method,and the component of serum protein by agarose gel electrophoresis. Results HBsAg carrier showed no significant difference（P 〉 0. 05） with healthy person in the component of serum protein. However, significant difference（P 〈 0. 01 ） was observed between the serum protein components of the patients with hepatitis B and healthy person. Compared with that of patients positive in HBsAg, HBeAg and HBcAb,the Alb levels of patients positive in HBsAg, HBeAb and HBcAb decreased significantly, and their globulin-7 levels increased significantly. However, the globulin α1, α2 and β levels of the patients showed no significant diffeence （P 〉 0.05）. Conclusion The combination of serum protein electrophoresis and tests for total protein and albumin was benefit to the overall study on character of serum protein pattern of patients with hepatitis B and was of important reference value in the clinical diagnosis and treatment of hepatitis B.
出处
《中国生物制品学杂志》
CAS
CSCD
2006年第1期96-98,共3页
Chinese Journal of Biologicals
