摘要
目的:建立一种检测早孕妇女血浆中胎儿游离DNA的新方法。方法:从50例妊娠7、8、9周的孕妇外周血血浆中提取胎儿游离DNA,用实时荧光定量聚合酶链反应(FQ-PCR)技术检测其中的Y性别决定区(SRY)基因。结果:38例早孕孕男胎妇女中32例血浆中出现SRY基因,灵敏度84.21%;12例早孕孕女胎的妇女血浆中均未出现SRY基因,特异度100%。结论:用实时荧光定量PCR技术可用来检测早孕妇女血浆中的胎儿游离DNA,这对无创性早期产前基因诊断方法的建立具有重大意义。
Objective：To establish the new technology for detection of fetal cell-free DNA in maternal plasma at early pregnant stage. Methods：Fetal cell free DNA in maternal plasma was islated from 50 samples （range, 7-9weeks）. Fetal DNA in maternal plasma was measured using real-time fluorescence quantitative polymcrase chain rcaction（FQ-PCR）for the SRY gene on the Y chromosome. Results ：Thirty-eight women at early pregnant stage （range ,7-9 weeks） carried male fetuses. Thirty two samples of them were positive ,the sensitivity of the system reaches 84.21%. The results of FQ-PCR were negative in the 12 women who carried female fetuses. The specificity of the systemreaches 100%. Conclusion：Fetal cell-free DNA in maternal plasma at early pregnant stage was measured using realtime fluorescence quantitative polymerase chain reaction（FQ-PCR）. It can be very valuable for early non-invasive prenatal diagnosis of inherited disorders.
出处
《临床医药实践》
2006年第3期175-177,共3页
Proceeding of Clinical Medicine
关键词
实时荧光定量聚合酶链反应
胎儿游离DNA
母体血浆
Y性别决定区基因
real-time fluorescence quantitative polymerase chain reaction
fetal cell free DNA,maternal plasma
sex-determining region Y gene
