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RNA聚合酶Ⅱ同BAF复合物和转录因子NF1/CTF之间的联系 被引量：2

The relation of RNA polymerase Ⅱ and BAF complex with transcription factor NF1/CTF
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摘要 通过免疫荧光共定位实验,研究了RNA聚合酶Ⅱ的亚基Rpb1,Rpb6,Rpb8与BAF复合物和转录因子NF1/CTF在基因转录活动中的联系.实验证实,RNA聚合酶Ⅱ的亚基Rpb1与BAF复合物有联系,Rpb6与BAF复合物联系不紧密,Rpb6与NF1/CTF联系密切,Rpb8与BAF复合物和NF1/CTF有部分联系.免疫沉淀实验也证实RNA聚合酶Ⅱ与BAF复合物和NF1/CTF一同参加了转录起始复合物的形成. This paper providest the evidences that the subunit Rpbl, Rpb6, Rpb8 of RNA polymerase Ⅱ have relationship with BAF complex by Immunofluorescene co - orientation assay . Tests validate the sub- unit Rpbl of RNA polymerase Ⅱ have relationship with BAF complex, the small subunit Rpb6 of RNA polymerase Ⅱ have no obvious orientation relationship with BAF complex, then the small subunit Rpb6 of RNA polymerase Ⅱ has obvious orientation relationship with transcription factor NF1/CTF, the small subunit Rpb8 of RNA polymerase Ⅱ have a little orientation relationship with BAF complex and NF1/CTF. The results of Immunoprecipitation assay further demmonstrates RNA polyrnerase Ⅱ and BAF complex with transcription factor NF1/CTF take participate in formation of transcriptional initiation complex together.
作者 赵丽辉 姜革强 周洪雷 邓国忠 王丽
机构地区 东北师范大学遗传与细胞研究所 吉林体育学院体育系 吉林大学物理学院
出处 《东北师大学报（自然科学版）》 CAS CSCD 北大核心 2006年第1期103-107,共5页 Journal of Northeast Normal University(Natural Science Edition)
基金 吉林省科技发展计划项目(20010710)
关键词 BAF复合物 转录因子NF1/CTF RNA聚合酶Ⅱ BAF complex transcription factor NF1/CTF RNA polymerase Ⅱ
分类号 Q813.4 [生物学—生物工程]
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参考文献10

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同被引文献23

  • 1唐冬生,严霞,张勇,张细权,李芳,蒋泓,李月琴,周天鸿.高GC含量的鳜鱼rRNA基因家族的克隆[J].中国生物工程杂志,2005,25(8):60-64. 被引量：10
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东北师大学报（自然科学版）
2006年 第1期

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