摘要
目的探讨端粒酶RNA(hTR)和反转录酶(hTERT)在银屑病皮损中的表达情况以及它们与角质形成细胞增殖关系。方法应用原位杂交方法检测银屑病皮损hTR、hTERTmRNA的表达,应用免疫组织化学方法检测Ki-67抗原表达,并对hTR、hTERTmRNA的表达与Ki-67抗原表达进行相关性分析。结果hTR表达在基底细胞、棘细胞、颗粒层细胞和所有有核细胞的胞浆,与细胞的增殖情况无关;其表达强度和阳性细胞百分率各组之间无明显差异(P>0.05)。而hTERT的表达与细胞增殖状态有关,主要表达在基底细胞层和棘细胞层下方,表达强度和阳性细胞百分率要明显高于正常皮肤(P<0.05)。Ki-67抗原主要表达在细胞生发层和分裂旺盛的组织;它的表达与hTERT的表达呈正相关(r=0.674,P<0.01);而与hTR(r=-0.295,P>0.05)无关。结论hTERTmRNA在银屑病皮损中的表达明显升高,与细胞增殖活性有关。hTR的表达和细胞增殖活性无关。
Objective To explore the expression of human telomerase RNA （hTR） component and telomerase reverse transcriptase （hTERT） mRNA in lesions of psoriasis vulgaris and their relationships with keratinocytes proliferation. Methods hTR and hTERT mRNA expression in skin were examined by in situ hybridization analysis. By contrast, sequential sections were stained by immunohistochemistry with anti-Ki-67 antibody, a proliferative marker. Results hTR were posiiive at moderate to strong levels in almost all basal, prickle, and granular cells of the epidermis. There was no significant difference between normal and lesional skin （P〉0.05）. However, the expression of hTERT mRAN was much higher in psoriasis than that in normal controls （P〈0.05）. It was found that the expression of Ki-67 in psoriasis was much stronger than that of healthy controls and statistically correlated with hTERT （r=-0.674, P〈0.01）, but not with hTR （r=-0.295, P〉0.05） expression. Conclusion Our results indicate that telomerase hTERT, rather than hTR, could be up-regulated in psoriatic lesions and should be represented as a new marker as Ki-67 for cell proliferation.
出处
《中国中西医结合皮肤性病学杂志》
CAS
2006年第1期5-7,共3页
Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine
