摘要
目的探讨胃癌细胞-树突状细胞(dendritic cell,DC)融合疫苗激活杀伤性T淋巴细胞的效应特点,为胃癌的免疫治疗提供实验基础与理论依据。方法(1)胃癌患者外周血单个核细胞经GM-CSF,IL-4,TNF-α诱导分化获取成熟DC。(2)SGC 7 9 0 1胃癌细胞-DC经PEG诱导融合、HAT/HT筛选培养,获得纯净融合细胞。(3)混合淋巴细胞反应对融合细胞疫苗激活效应T淋巴细胞的增殖能力进行检测。结果按上述方法,可获得具备典型特征的DC及纯净的融合细胞。融合疫苗显著刺激T淋巴细胞增殖,与T淋巴细胞在1∶1效靶比时刺激能力最强,与对照组比较差异非常显著(P<0.0 1)。结论胃癌细胞-DC融合疫苗具有较亲代DC更强的刺激T淋巴细胞增殖的能力,可能是其发挥更强抗肿瘤生物学效应的基础。
Objective To study the activation effects to cytotoxic T lymphocytes by gastric cancer cell-dendritic cell （ DC ） fusion vaccines and to provide theoretical data for biological therapy of gastric cancer based on anti-tumor fusion vaccines. Methods （1）Peripheral blood mononuclear cells from gastric cancer patients were separated and co-cultured with granulocyte macrophage colony stimulating factors, interleukin-4 and tumor necrosis factor-et to generate mature dendritic cells. （2）The dendritic cells and SGC7901 cells were fusioned by use of polyethylene glycol, and the pure fusion cells were screened out and cultured with HAT and HT selective culture systems. （3） The ability of fusion cells to activate the cytotoxic T lymphocytes was investigated by using MLA method. Results （1）Mature dendritic cells were gained from gastric cancer patients＇ peripheral blood mononuclear cells by co-culturing with granulocyte-macrophage colony stimulating factors, interleukin-4 and tumor necrosis factor-α. （2）Dendritic cells and SGC7901 cells could be fusioned by PEG and could get pure fusion cells by culture with HAT/HT selective culture systems. （3）The fusion vaccines could strongly activate cytotoxic T lymphocytes, and there were significent differences between the fusion vaccine group and the control group. （ P 〈 0. 01 ） . Conclusions Gastric cancer cell-dendritic cell fusion vaccine has stronger ability to stimulate the proliferation of T lymphocytes than that of parent dendritic cells, and may be a basis for induction of stronger anti-tunmor biological effects.
出处
《中国普通外科杂志》
CAS
CSCD
2006年第2期94-97,共4页
China Journal of General Surgery
