摘要
目的 研究高效液相色谱快速测定辣椒粉中苏丹红Ⅰ号的实际应用方法，探讨测定过程中干扰的来源及消除的方法，为提高定性定量的准确度和可靠性提供依据。方法 建立该技术的应用方法，对操作参数进行优化，测定和评价方法学指标，实际检测辣椒粉中苏丹红Ⅰ号含量并进行验证，同时与国标法进行比较。结果 本法标准曲线线性范围：0～100μg／ml；线性相关系数：r=0．9999；最低检出浓度为0．103μg／ml；RSD=1．03％-3．37％；平均回收率95．3％～96，7％。结论 采用本文建立的高效液相色谱快速测定辣椒粉中苏丹红Ⅰ号的应用方法，避免了国标法中的一些不足之处所引起的多种干扰，较国标法操作简便，扩大了测定的线性范围，提高了定性、定量分析的准确度和可靠性，实验快速易行，实用性强。
Objective To studya rapid test for Sudan 1 in chili powder with HPLC and discuss the interference source and the elimination of the source in the test so as to improve the accuracy and reliability in qualitative and quantitative. Methods The rapid test for Sudan 1 with HPLC was established. The operating parameters were optimized. The test indexes were determined and evaluated. Then, Sudan 1 in the chili powder was examined. Meanwhile, a comparison with international standard method was made. Results The standard curve linear range with this method was 0 ～100μg/ml, the linear coetticient ：r = 0.9999, the lowest concentration detected was 0. 103 μg/ml,RSD = 1.03 % -3.37 %, the average recovery of Sudan 1 was 95.3% - 96.7%. Conclusion This rapid test for Sudan 1 in chili powder avoided muhi - interference caused by some deficiencies of international standard. It was also easier to operate compared with international standard method. This method widened the linear range in determination and improved the accuracy and reliability in qualitative and quantitative, which made the experiment easier, faster and more applicable.
出处
《安徽预防医学杂志》
2006年第2期93-95,共3页
Anhui Journal of Preventive Medicine
关键词
苏丹红Ⅰ
高效液相色谱
快速测定
Sudan Ⅰ
High Performance liquid chromatography
rapid test
