摘要
目的观察卡托普利预处理对缺氧复氧乳鼠心室肌细胞游离钙和钠钙交换电流(Na+/Ca2+exchange current,INa-Ca)的影响。方法建立培养乳鼠心肌细胞缺氧/复氧损伤模型。Flou-3/AM负载染色,应用流式细胞分析技术,测定细胞内钙离子浓度([Ca2+]i);利用全细胞膜片钳技术,观察INa-Ca的变化。结果与正常对照组比较,缺氧复氧可显著增加[Ca2+]i和INa-Ca。与缺氧复氧组比较,卡托普利呈浓度依赖性抑制缺氧复氧时[Ca2+]i增加,减少INa-Ca的增加。但[Ca2+]i和INa-Ca仍高于正常对照组。结论心肌细胞缺氧复氧可引起[Ca2+]i的异常升高,与INa-Ca的异常增加有关;卡托普利预处理可能通过轻度增加INa-Ca及其[Ca2+]i而触发心脏延迟保护作用,抑制后续缺氧复氧引起的INa-Ca及其[Ca2+]i的异常增加。
OBJECTIVE To observe the influence of captopril on in tracellular free calcium concentration （[Ca^2＋]i） and Na^＋/Ca^2 ＋ exchange current in the cardiac myocytes of the neonatal rat undergone anoxia-reoxygenation injury.METHODS The anoxia-reoxygenation models were developed. The cultured ventricular myocytes of neonatal rat were established and divided into normal, anoxia-reoxygenation and captopril preconditioning groups. By using Flou-3/AM loading and flow cytometry tectmique, the [Ca^2＋]i was observed, and whole-cell patch clamp technique was used to record the Na^＋/Ca^2＋ exchange current. RESULTS ①Compared with normal group, [Ca^2＋]i and Na^＋/Ca^2＋ exchange current in anoxia-reoxygenation group were increased significantly （ P 〈 0.01 ）. ②Compared with anoxia-rcoxygcnation group, the captopril precondition group resulted in a significant decrease of [Ca^2＋]i in a concentration dependent manner, and Na^＋/Ca^2＋ exchange current was also decreased. ③Compared with normal oxygen condition, the captopril precondition group resulted in a light increase of [Ca^2＋]i and Na^＋/ Ca^2＋ exchange current. CONCLUSION The anoxia-reoxygenation injury in cardiac myocytes resulted in an abnormal increase of [Ca^2＋]i by increasing Na^＋/Ca^2＋ exchange current.late preconditioning in cardiac myocytes is triggered by captopril which slightly increased Na^＋/Ca^2＋ exchange current and [Ca^2＋]i, and restrained the abnormal increasing of Na^＋/Ca^2＋ exchange current and calcium overload induced by subsequenced anoxia-reoxygenation injury.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2006年第4期275-278,共4页
Chinese Pharmaceutical Journal
基金
吉林省科技发展计划资助(吉发合字990581-1)
关键词
卡托普利
缺氧复氧
心室肌细胞
游离钙
钠钙交换电流
captopril
anoxia-reoxygenation
cardiac myocytes
free calcium concentration
Na^＋/Ca^2＋ exchange current
