摘要
目的探讨脱氢表雄酮(DHEA)对成骨细胞(osteoblasts,OB)及CD4+T细胞表达协同刺激分子的调控作用。方法颅骨酶解法培养鼠OB,体外模拟雌激素撤退;免疫磁珠细胞分选(mag-netic cell sorting,MACS)法分离CD4+T细胞;将OB或CD4+T细胞分为对照组、E2及DHEA处理组,并以LPS刺激;以流式细胞术分析OB表面CD80、CD86以及CD4+T细胞表面CD28、CTLA-4的表达。结果经E2及DHEA处理后,OB表达CD80、CD86显著增加(P<0.05,P<0.01);CsA可降低对照组及DHEA处理组OB CD80、CD86的表达(P<0.01)。除DHEA组CD28+T细胞百分比增加外(P<0.05),其余各组CD4+T细胞CD28和CTLA-4的表达无显著改变(P>0.05)。结论DHEA可上调鼠OB协同刺激分子CD80、CD86表达,该作用可被CsA阻滞;DHEA还上调CD4+T细胞CD28的表达,提示可改善骨-免疫调节网络。
Objective To investigate modulation of DHEA in the costimulatory molecule of the osteoblasts（OB） and CD4^＋ T lymphocytes. Methods The OBs were isolated from calvaria of neonatal BALB/c mice, and cultured by the enzyme-digested method. The CD4^＋ T cells were isolated from spleen with MACS separation. The OBs or CD4^＋ T ceils were treated with E2 or DHEA and then stimulated by LPS. rI＇ne expression of costimulatory molecules on the OBs and CD4^＋ T lymphocyte were examined by flow cytometry（ FCM） . Results The expression of the costimulatory molecule CD80, CD386 on the OBs significantly increased after treatment with E2 or DHEA（ P 〈0.05 or P 〈0.01）. The expression of the costimulatory molecules like CD80, O386 on OBs in the DHEA-treating group and in the control were suppressed significantly by CsA in vitro（ P 〈 0.01）. DHEA significantly increased the expansion of the CD4^＋ CD28^＋ T cells（ P 〈 0.05）. Conclusion DHEA up-regulates the expression of costirnulatory molecule CD80 and CD86 on OBs, that can be blocked by CsA. DHEA also inca＇eases the expansion of the CD4^＋ CD28^＋ ceils, and likely improves the osteo-inunune network.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2006年第2期110-114,共5页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金(30472259)
上海市科技攻关项目(004019061)资助项目
