摘要
目的探讨心肌细胞移植对Wistar大鼠心肌梗死后左室重构及心功能的影响。方法50只雄性Wistar大鼠结扎冠状动脉左前降支成功建立慢性心肌梗死模型,随机分为试验组,即新生鼠心肌细胞移植组25只;对照组,即细胞培养液基质移植组25只。另设假手术组15只为正常对照。心肌梗死后4周和细胞移植后4周,采用高频超声心动图评价大鼠左室形态和功能。用免疫组化技术检测试验组移植细胞的存在。结果细胞移植后4周,与对照组相比,试验组左室收缩末期内径、舒张末期内径、收缩末期容积和舒张末期容积均显著缩小(P<0.01),左室前壁舒张末期厚度明显增厚(P<0.01),左室射血分数和缩短率显著升高(P<0.01)。免疫组化显示试验组心肌瘢痕区边缘有BrdU阳性细胞存在。结论心肌细胞移植可逆转心肌梗死大鼠左室重构,改善左室功能。高频超声心动图为心肌细胞移植效果评价提供了有效实用的方法。
Objective To evaluate the effect of cardiomyocyte transplantation on left ventricular （LV） remodeling and function in Wister rats with chronic myocardial infarction. Methods Chronic myocardial infarction was induced in 100 Wister rats by ligating the left anterior descending artery. Four weeks later, fifty rats were used who were randomized into two groups： one group that received neonatal cardiomyocyte transplantation （trial group,25 cases）; another group that received culture medium only （control group,25 cases）. Fifteen rats were sham-operated group. The shape and function of LV were evaluated by high frequency ultrasound before and four weeks after cell transplantation. After the final evaluation,all rats were sacrificed for histological study. Results Four weeks after cell transplantation, compared with the values in the control group, the LV end-systolic dimension,end-diastolic dimension,end-systolic volume and end-diastolic volume decreased （ P 〈0. 01 ）, and the LV anterior wall end-diastolic thickness,LV ejection fraction and fractional shortening increased （ P 〈0.01） in the trial group. Histological study showed that transplanted neonatal cardiomyocyte were detected in all host hearts and identified by BrdU staining. Conclusions Neonatal cardiomyocyte transplantation can reverse cardiac remodeling and improve heart function in chronic myocardial infarction rats. High frequency ultrasound can be used as a reliable technique to evaluate the effect of cardiomyocyte transplantation.
出处
《中华超声影像学杂志》
CSCD
2006年第3期216-219,共4页
Chinese Journal of Ultrasonography
关键词
超声心动描记术
心肌梗塞
干细胞移植
心室功能
左
Echocardiography
Myocardial infarction
Stem cell transplantation
Ventricular function,left
