摘要
目的研究急性缺血再灌注肾损伤(I/R)的机制及胡黄连提取物对I/R的防治作用。方法雄性SD大鼠37只,分为假手术组(5只)、手术对照组(8只)、不同剂量(40、80、160mg/kg)胡黄连提取物给药组(各8只),采用切除右肾后,用无创动脉夹夹闭左肾动脉60min再灌注72h的方法制备急性缺血再灌注肾损伤大鼠模型,测定血肌酐、尿肌酐,结合尿量计算肌酐清除率(Ccr),光镜、电镜观察肾组织形态学并进行肾小管计分,硫代巴比妥酸比色法测定血清丙二醛(MDA),改良DTNB显色法测定谷胱甘肽过氧化物酶(GPx),ELISA法测定血清肿瘤坏死因子α(TNF-α)。结果肾脏I/R损伤时大鼠肾功能明显减退,肾脏病理变化明显,血清MDA、TNF-α升高,GPx下降;与手术对照组相比,各胡黄连提取物给药组肾小管计分降低(P<0·01或P<0·05),血清MDA降低(P<0·01或P<0·05),TNF-α降低(P<0·05),GPx含量升高(P<0·01),胡黄连提取物80mg/kg给药组和160mg/kg给药组Ccr升高(P<0·01)。结论胡黄连提取物可以减轻急性缺血再灌注肾损伤,促进肾功能恢复,此作用可能与其抗氧化、抑制炎症反应有关。
Objective To investigate the protective ettect ot extract ot Picrhoriza scrophulariflora on renal ischemia/reperfusion injury（I/R）. Methods Male SD rats were randomly divided, into sham-operated group （n=5）, I/R group（n=8）, I/R＋extracts of Picrhoriza scrophulariflora groups （each group had 8 rats）. Acute renal ischemia/reperfusion injury in rats was reproduced by removing the right kidney and clamping the left renal artery with a nor-traumatic vascular clamp for 60min followed by reperfusion for 72h. Serum creatinine, urine creatinine and renal pathological changes, were compared between I/R and I/R ＋ extracts of Picrhoriza scrophulariflora groups, malondialdehyde （MDA） and glutathione peroxidase （GSHPx）. Results CCr levels were significant decreased after renal I/R injury. Kidneys of animal with I/R injury displayed significant pathological changes. 72h after ischemia, Ccr and serum GSHPx of animals of the I/R＋ extracts of Picrhoriza scrophulariflora group were significantly higher than those of I/R group（P〈0. 01 or P〈0. 05）. Serum levels of MDA and TNF-a in animals of the I/R ＋ extracts of Picrhoriza scrophulariflora group were significantly lower than those of I/ R group （P〈0.01 or P〈0.05）. The pathogical changes in kidneys of animal of the I/R ＋ extracts of Picrhoriza scrophulariflora group was milder than that of I/R group. Conclusions Extracts of Picrhoriza scrophulariflora protect kidney against ischemia/reperfusion injury and accelerate recovery after the injury, and the effect may be attributable to the anti-oxidation and anti-inflammation actions of extracts of Picrhoriza scrophulari flora.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2006年第3期237-239,共3页
Medical Journal of Chinese People's Liberation Army
关键词
胡黄连提取物
再灌注损伤
肾
extracts of Picrorhiza scrophlariiflora Pennell
repelfusion injury
kidney
