摘要
目的:观察脑出血后血肿周围半胱氨酸天冬氨酸蛋白酶3的表达,及尼莫地平对其的影响。方法:实验于2004-07在大连医科大学中心实验室进行。取120只SD雄性大鼠随机分为3组:①尼莫地平组(n=50):尾壳核注射自体股动脉血50μL复制脑出血模型,造模即刻腹腔注射尼莫地平1.6mg/kg(即8μL/g),以后每天1次。②模型组(n=50):同前造模,术后腹腔注射等量生理盐水。③假手术组(n=20):手术,但进针入尾壳核后不注血。各组分为术后6,24,48,72h、5d5个时间点。造模动物醒后进行Bederson评分,评估其行为和神经功能缺陷(0～3分,评分越高,神经功能缺陷越重,评分≥2分为造模成功)。入组动物在以上5个时间点进行Bederson评分后,麻醉状态下处死取脑,经尾壳核行冠状切片,行免疫组化测定半胱氨酸天冬氨酸蛋白酶3表达。结果:80只大鼠进入结果分析。①Bederson评分:模型组、尼莫地平组大鼠醒后迅速出现偏瘫,24h后评分趋于稳定,至72h之间评分最高,然后逐渐下降,5d时仍有体征。模型组、尼莫地平组各时间点评分均高于对照组(P<0.01),尼莫地平组术后48,72h评分低于模型组(P<0.05)。②血肿周围组织半胱氨酸天冬氨酸蛋白酶3表达:假手术组进针侧脑组织表达很少,模型组6h后即有表达,24h达高峰,持续72h后逐渐下降,5d后仅有少量表达;尼莫地平组动态变化趋势与模型组相同,但各时间点的数值均较低,尤其是术后24～72h(P<0.01)。结论:①脑出血后血肿周围组织中半胱氨酸天冬氨酸蛋白酶3表达升高,提示其与脑出血血肿周围组织损伤有一定关系。②尼莫地平降低脑出血后血肿周围组织中半胱氨酸天冬氨酸蛋白酶3表达,从而减轻细胞凋亡程度,对神经细胞起到保护作用,降低神经功能缺陷。
AIM： To observe the expressions of caspase-3 in the perihematomal tissues of rats after intracerebral hemorrhage （ICH） and study the influence of nimodipine on the expressions. METHODS： The experiment was conducted at the Center Laboratory of Dalian Medical University in July 2004. Totally 120 male SD rats were randomly divided into 3 groups： ①Nimodipine group （n=50）： 50μL autologous femoral arterial blood was injected into caudate nucleus to duplicate ICH models, which were instantly given 1.6 mg/kg （8μL/g） nimodipine injection intraperitoneally, once a day.②Models group （n=50）： The model establishments were performed as above, and same dose of saline were injected intraperitoneally after operation. ③Sham-operated group （n=20）： After the operation, the needle was only inserted into caudate nucleus without blood injection, and then drawn out. Each group was further divided into 5 time points （6,24,48,72 hours and 5 days）, respectively. The behavior and neurologic impairments were evaluated after model animals awaked, with Bederson score （scored from 0 to 3, the higher score indicated the severer neurologic impairment, and the model was established successfully if the score was more than 2）. After evaluated by Bederson score at the above 5 time points respectively, all the experimental animals were executed to take out the brains in anesthetized state. Coronal section via caudate nucleus was performed. The expressions of caspase-3 were assayed with immunohistechemical method. RESULTS： Totally 80 rats were involved in the result analysis.①Bederson score： In the model and nimodipine groups, hemiplegia appeared instantly after the model animals awaked. The score showed stable after 24 hours, peeked at 24-72 hours, then declined, remained certain levels the 5 days. The scores of 5 time points were higher in the model group and nimodipine group than in the control group （P 〈 0.01）. The scores of 48, 72 hours after operation in nimodipine group were lower than those of model group （P 〈 0.05）. ② The expressions of caspase-3 in the perihematomal tissues： After the needle insertion, the positive cells of caspase-3 expressed rarely in the sham-operated group. However, in the model group, the caspase-3 began to express 6 hours later, peeked at 24 hours, remained a certain level till 72 hours, and then declined, finally expressed a little after 5 days. The nimodipine group was similar in development tendency to the model group, but the＇expressions at each time point were lower than those in the model group, especially at 24-72 hours after operation （P 〈 0.01）. CONCLUSION： ① The high expressions of caspase-3 in the perihematomal tissues after ICH show that easpase-3 is associated with the injury of perihematomal tissues.②Nimodipine can reduce the expression of caspase-3 in the perihematomal tissues after ICH, thus decrease the level of cell apoptosis, protect the neurons after ICH and relieve the neurologic impairment.
出处
《中国临床康复》
CSCD
北大核心
2006年第12期110-112,共3页
Chinese Journal of Clinical Rehabilitation
