摘要
目的:研究Th1和Th2型细胞因子在胎盘淋巴细胞内的表达状况及其与NOD/SCID孕鼠妊娠转归的关系。方法:比较BALB/c和NOD/SCID小鼠孕13.5d胚胎吸收率(RR),采用4色流式细胞术检测胎盘淋巴细胞内Th1型(TNF-α和IL-2)和Th2型(IL-10)细胞因子表达率。结果:虽然NOD/SCID小鼠存在多重免疫缺陷,但BALB/c和NOD/SCID小鼠孕13.5dRR无显著差异;NOD/SCID×NOD/SCID母-胎界面CD8+IL-10+/CD8+细胞百分率显著高于BALB/c×BALB/c妊娠模型(P<0.01),而CD4和CD8阳性细胞内TNF-α和IL-2表达水平均无显著差异。结论:母-胎界面CD8+IL-10+/CD8+细胞百分率自发性升高可能与NOD/SCID小鼠基本正常的生育力有关。
AIM：To investigate the relationship between the intracellular expreasion of Th1 and Th2 type cytokines in placental lymphocytes and the pregnancy outcomes in NOD/SCID mice.METHODS：The resorption rate of embryos（RR） was compared between BALB/c and NOD/SCID mice.In addition,the expression rates of Th1 type （TNF-α and IL-2） and Th2 type （IL-10） cytokines were detected intracellularlly in placental lymphocytes by four-color flow cytometry.RESULTS：A lthough multiple-immunodeficiency was confirmed in the NOD/SCID,no significant difference was observed in RR between BALB/c and NOD/SCID mice.At the same time,a dramatically elevated level of CD8^＋ IL-10^＋/CD8^＋ cell percentage was found at the feto-maternal interface in NOD/SCID×NOD/SCID,as compared with BALB/c×BALB/c mice,while no significant difference was observed for TNF-α and IL-2 expression in CD4^＋ and CD8^＋ cell subsets isolated from these mice.CONCLUSION：The spontaneous for TNF-α and IL-2 expression in CD4^＋ and CD8^＋ cell subsets isolated from these mice.CONCLUSION：The spontsneous elevation of CD8^＋ IL-10^＋/CD8^＋ cell percentage at the feto-matemal interface may be related to the roughly normal fertility in NOD/SCID mice.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第3期563-566,共4页
Chinese Journal of Pathophysiology
基金
暨南大学引进优秀人才科研启动基金资助(No.51204067)
广东省自然科学基金资助(No.04300213)
中国博士后科学基金资助(No.2002032244)
关键词
胎盘
细胞因子类
免疫缺陷
妊娠结局
Placenta
Cytokines
Immunodeficiency
Pregnancy outcome
