摘要
目的:研究肾上腺素对脂多糖(LPS)诱导的小鼠单核巨噬细胞株RAW264.7中促炎介质[肿瘤坏死因子(TNF-α)、一氧化氮(NO)、环加氧酶-2(COX-2)]和抗炎介质[血红素氧化酶-1(HO-1)、白介素10(IL-10)]表达及NF-κB活化的影响。方法:以10μg/L的LPS刺激体外培养的RAW264.7细胞作为炎症模型,加入不同浓度的肾上腺素(1、5、10、50μmol/L)孵育24h后,收集培养上清并提取细胞总蛋白,酶联免疫法测定上清中TNF-α、IL-10浓度,Griess法检测上清NO含量(以NO2-/NO3-表示),免疫印迹法检测细胞总蛋白中COX-2、HO-1、IκB-α的含量。结果:10μg/L的LPS明显诱导TNF-α、NO(NO2-/NO3-)、COX-2、IL-10及HO-1的产生;LPS+肾上腺素组与LPS单独作用组相比促炎介质TNF-α、NO(NO2-/NO3-)、COX-2的表达量显著下降,而抗炎介质IL-10、HO-1的表达却明显增强;肾上腺素与LPS共同作用组中IκB-α的含量与单独LPS作用组相比无明显差异。结论:肾上腺素下调LPS诱导的巨噬细胞中促炎介质的表达同时促进抗炎介质的表达,这种效应并不通过影响NF-κB的活化来实现。
AIM： To investigate the effect of epinephrine on LPS - induced pro - inflammatory mediators （TNF - α, NO and COX-2） and anti - inflammatory mediators （HO- 1 and IL- 10） production in murine macrophage RAW264.7 cells, and to determine whether these effect is due to the influence of epinephrine on NF - κB activation.METHODS： RAW264.7 cells were cultured in vitro with 10 μg/L LPS in the absence or presence of epinephrine at variant concentrations （1, 5, 10, 50μmol/ L） for 24 hours, then the supematants was collected for measuring TNF-α and IL- 10 by ELISA and Griess reagent was used to measure NO （NO2^-/NO3^- ） concentration. At the same time point, cells were harvested and COX - 2, HO - 1 and IκB - α was detected by Western blotting. RESULTS： 10μg/L LPS significantly induced the production of TNF - α, NO （NO2^-/NO3^- ）, COX - 2, HO - 1 and IL- 10. When epinephrine was added into the medium together with LPS, the pro - inflammatory mediators production was decreased in a dose- dependent manner, however, anti - inflammatory mediators HO- 1 and IL- 10 expression was enhanced by epinephrine. Epinephrine has no significant effect on IκB - α degradation in LPS - activated RAW264.7 cells. CONCLUSION： Epinephrine down- regulates LPS- induced pro- inflammatory mediator expression while promotes anti- inflammatory mediator production in routine macrophages. These effect seems to be independent of NF-κB activation.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第3期555-558,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30070929
No.30100226
No.30200373)
关键词
肾上腺素
脂多糖类
炎症
巨噬细胞
NF—κB
Epinephrine
Lipopolysaccharides
Inflammation
Macrophages
NF-kappa B
