摘要
目的:观察海马CA1区神经元在不同浓度海洛因灌流时电生理特性的变化。方法:运用神经元胞内记录技术,对大鼠离体海马脑片给予3种浓度海洛因(heroin)急性灌流,分别记录CA1区神经元灌流前、后电生理参数,进行对比。结果:随着海洛因灌流浓度的增加,神经元静息电位和阈电位绝对值小于灌流前(P<0.05)。0.3mmol·L-1灌流时,动作电位振幅低于灌流前(P<0.05),锋电位半幅时程,10%-90%上升时间和10%-90%衰减时间延长(P<0.05,P<0.01)。0.1-0.3mmol·L-1灌流时,电流-电压(I-V)关系曲线显示膜斜率电阻显著高于灌流前(P<0.05,P<0.01)。电流-频率(I-F)关系曲线表明,0.03mmol·L-1海洛因灌流15min时,0.1-1.8nA胞内电流刺激,神经元放电频率与对照相似,但1.9-2.0nA刺激时可保持较高放电频率(P<0.05)。3种浓度海洛因对神经元兴奋性突触后电位(excitatorypostsynapticpotential,EPSP)均有抑制作用,以0.1-0.3mmol·L-1明显。结论:不同浓度海洛因对海马CA1区神经元膜电特性具有显著影响。
AIM： To investigate changes of electrophysiological properties of rat hippocampal CA1 pyramidal neurons in perfusion with heroin at different concentrations. METHODS： The intracellular recordings were made from CA1 pyramidal neurons of isolated hippocampal slices in perfusion with 0.03-0.3 mmol·L^-1 heroin. Compared with control parametes before herion perfusion, the electrophysiological data obtained from identical neurons at 15 min after acute perfusion of the different concentration heroin were analyzed. RESULTS： With the increase of the heroin concentrations in perfusion, the absolute values of neuron resting potential and threshold potential decreased （ P 〈0.05）, In 0.3 mmol·L^-1 heroin perfusion group,the amplitude of actian potential dacreased （P 〈 0.05）, 10% -90% risefime and 10% -90% decaytime of spike potential elongated （ P 〈0.05, P 〈 0.01）, and I- V relation curve indicated membran slope resistance increased in 0.1 -0.3 mmol·L^-1 perfusion group （P 〈 0.05, P 〈 0.01）. I - F relation curve showed that neuron average firing frequency reeored at 15 min after 0.03 mmol·L^-1 heroin infusion was similar to the control frequency when intracellular stimulus intensity was 0.1 - 1.8 hA, however, firing frequency kept the higher level （ P 〈 0.05, P 〈 0.01 ） in 1.9- 2.0 nA vs the control frequenee. It was evident that EPSPs of pyramidal neurons were inhibited in perfusion of three concentration heroin, especially in 0.3 mmol·L^-1. CONCLUSION： These results indicate acute heroin perfusion has a significantly influence on electrical activities of hippocampus CA1 pyramidal neurons.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第3期528-531,共4页
Chinese Journal of Pathophysiology
基金
安徽省教育厅自然科学研究基金资助(No.2000J1238)
皖南医学院中青年科研基金资助(No.WK200203)
关键词
海洛因
海马
电生理学
Heroin
Hippoeampus
Electrophysiology
