摘要
目的:观察氟伐他汀对大鼠心肌梗死后左室重构及凋亡基因caspase-3的影响。方法:复制心肌梗死动物模型,实验分为4组,Ⅰ组假手术组,Ⅱ组假手术+氟伐他汀组,Ⅲ组模型组,Ⅳ组模型+氟伐他汀组。Ⅱ和Ⅳ组术后给氟伐他汀10mg·kg-1·d-1共4周,观察左室结构及心动超声变化,心肌羟脯氨酸含量及caspase-3表达。结果:Ⅳ组心肌超微结构和左室重构指标改善程度明显高于Ⅲ组,羟脯氨酸含量及免疫组化caspase-3阳性细胞明显少于Ⅲ组(P<0.05),RT-PCRcaspase-3mRNA表达也明显少于Ⅲ组(P<0.05)。结论:氟伐他汀能改善大鼠心梗后左室重构,并下调凋亡基因caspase-3表达,抑制细胞凋亡。
AIM： To observe the effect of fluvastatin （FV） on left ventricular remodeling and expression of caspase - 3 after myocardial infarction （MI） in rats. METHODS： Rats were divided into 4 groups： group Ⅰ （sham）, group Ⅱ （sham＋ FV）, group Ⅲ （MI） and group Ⅳ （MI＋ FV）. group Ⅱ and Ⅳ were treated with FV （10 mg·kg^-1·d^-1） for 4 weeks. The left ventricular structure, echocardiography and hydroxypmline were observed. The expression of caspase- 3 was measured by immuno-histochemistry and RT- PCR. RESULTS： Compared with MI group, there was a improvement of ultrastructure and index of left ventricular remodeling, and decrease in hydroxypmline in MI ＋ FV group （all P 〈 0.05）. The number of caspase - 3 positive cells also decreased in MI ＋ FV group, and RT - PCR showed the level of caspase - 3 mRNA expression was lower than that in MI group （ P 〈 0.05）. CONCLUSION： Fluvastatin improves left ventricular remodeling after myocardial infarction, decreases the expression of caspase- 3 and inhibits apoptesis.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第3期472-476,共5页
Chinese Journal of Pathophysiology
基金
黑龙江省科学技术厅资助项目(No.GC04C30106)
