摘要
目的:观察缺氧预适应对培养乳鼠窦房结细胞骨架结构完整性的影响。方法:取培养2d的乳鼠窦房结细胞,随机分为对照组、缺氧/复氧(H/R)组及缺氧预适应(HP)组,通过免疫荧光法分别标记微丝(F-actin)、纽带蛋白(vinculin)、微管蛋白(β-tubulin)及结蛋白(desmin),以激光共聚焦显微镜检测各组细胞骨架结构的改变。结果:①H/R组窦房结细胞的4种骨架蛋白量明显低于对照组(P<0.01),细胞骨架结构破坏显著,细胞变形,胞膜破损。②HP组骨架蛋白量虽仍与对照组有明显差异,但显著高于H/R组(P<0.01),细胞骨架结构维护相对较好,细胞形态较规则,胞膜基本完整。结论:HP能够减轻H/R引起的乳鼠窦房结细胞骨架结构损伤,维持细胞骨架的相对完整性。
AIM： To evaluate the effect of hypoxic preconditioning （HP） on the cytoskeleton of sinoatrial node cells primarily cultured from neonatal rat. METHODS： Cells were randomized to three groups of control, hypoxia/reoxygenation （H/R） and HP. F - actin, vinculin, β- tubulin and desmin were studied with laser confocal microscopy after labled with different immune fluorescent agents. RESULTS： Compared with control, although both H/R and lip reduced the fluorescence intensity of these four cytoskeletal proteins remarkably （ P 〈 0.01 ）, and destroyed the normal cytoskeletal structure, HP otherwise enhanced significantly the fluorescence intensity of these cytoskeletal proteins as well as better maintained their structural integrity compared with H/R. CONCLUSION： This study demonstrates that lip relieves the cytoskeleton injury caused by H/R and maintains the relative integrity of cytoskeleton.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第3期448-451,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30070314)
关键词
窦房结
缺氧预处理
细胞骨架
Sinoatrial node
Hypoxic preconditioning
Cytoskeleton
