摘要
目的探讨汽油、甲醇燃烧汽车尾气的雌激素样作用,为甲醇取代汽油作为汽车燃料提供卫生学依据。方法采用环境雌激素基因重组酵母测评系统,分别测定汽油、甲醇燃烧汽车尾气的雌激素活性。结果汽油燃烧汽车尾气在高剂量1000mL/mL时吸光度值(A600)仅为0.081,与二甲亚砜(DMSO)对照组相比差异有统计学意义(P<0.05),说明汽油尾气在该剂量下对酵母细胞有毒性作用;汽油燃烧汽车尾气在31.25mL/mL剂量时检出雌激素活性,与DMSO对照组相比差异有统计学意义(P<0.05),其他各剂量组均未检出雌激素活性。甲醇燃烧汽车尾气各剂量组与DMSO对照组相比,A600差异均无统计学意义(P>0.05),也未检出雌激素活性。结论以汽油作为燃料的汽车尾气具有微弱雌激素样作用,以甲醇作为燃料的汽车尾气无雌激素样作用。
Objective To investigate the estrogenic effects of gasoline-fueled vehicle exhausts and methanol-fueled vehicle exhausts and provide a scientific basis for replacement of gasoline by methanol as fuel in vehicle. Methods The estrogen-like activities of gasoline-fueled vehicle exhausts and methanol-fueled vehicle exhausts were detected using the recombinant yeast estrogen system. Results The A600 value of gasoline-fueled vehicle exhausts was 0. 081 when the concentration of gasoline-fueled vehicle exhausts was 1000 mL/mL, and there were significantly statistical differences as compared with those of the dimethyl sulfoxide （DMSO） control group（P〈0. 05）, which indicated that such concentration had poisonous effects on yeast cell; when the concentration of gasoline-fueled vehicle exhausts was 31.25 mL/mL, the estrogen-like activities of gasoline-fueled vehicle exhausts could be detected, and there were significantly statistical differences as compared with those of the DMSO control group（P〈0. 05）. Besides, the estrogen-like activities could not be detected in other groups with various concentrations. The estrogen-like activities of methanol-fueled vehicle exhausts could not be detected, and there were no significantly statistical differences in A6oo value （P〉O. 05）when each group was compared with the DMSO control group. Conclusion Gasoline-fueled vehicle exhausts have weak estrogenic effects, whereas methanol-fueled vehicle exhausts have no such effects.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2006年第2期292-294,共3页
Journal of Sichuan University(Medical Sciences)
关键词
汽油燃料
甲醇燃料
环境雌激素
重组酵母测评系统
Gasoline-fueled vehicle exhausts Methanol-fueled vehicle exhausts Environmental estrogens Recombinant yeast estrogen system
